Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule
Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule
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DOI：
10.1021/acschembio.7b00647
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发表时间：
2017-10-01
影响因子：
4
通讯作者：
Okuma, Kentaro
中科院分区：
文献类型：
--
作者：
Iwashita, Hidefumi;Torii, Satoru;Okuma, Kentaro
线粒体自噬是一种选择性自噬，在维持细胞内环境稳定中起着重要作用。我们已经开发了一种小分子荧光探针，Mtphagy染料，用于可视化线粒体自噬，这是很容易从一个已知的苝衍生物，苝-3，4-二羧酸酐合成。Mtphagy Dye具有合适的荧光特性，用于在不损伤线粒体的长波长区域中检测线粒体自噬期间的线粒体酸化。使用Mtphagy染料，我们能够可视化与帕金森依赖性和非依赖性HeLa细胞的情况下的线粒体自噬。
There has been a growing interest in mitophagy, mitochondria-selective autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize mitophagy with both cases of Parkin-dependent and -independent HeLa cells.