EVIDENCE FOR CAPPING OF FC-GAMMA-RECEPTORS ON OSTEOCLASTS
EVIDENCE FOR CAPPING OF FC-GAMMA-RECEPTORS ON OSTEOCLASTS
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DOI：
10.1007/bf02553299
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发表时间：
1986-08-01
影响因子：
4.2
通讯作者：
LINDSKOG, S
中科院分区：
文献类型：
--
作者：
PIERCE, AM;LINDSKOG, S
Fc受体，结合免疫球蛋白Fc部分的细胞表面结构，促进内吞作用并介导单核吞噬细胞系统（MPS）吞噬细胞中触发的酶释放。破骨细胞和巨噬细胞有许多相似之处，并且已经表明它们都来源于共同的MPS前体细胞。然而，Fc γ。在单核吞噬细胞系统的原始细胞和分化良好的细胞上发现的受体，在破骨细胞膜上还有待证实。本研究的目的是第一，研究大鼠内源性IgG的分布周围再吸收骨使用免疫组织化学和抗生物素蛋白-生物素-过氧化物酶（ABC）技术;和第二，以确定大鼠破骨细胞是否表达Fc。受体，使用最近描述的基于ABC方法的技术。观察到内源性IgG在破骨细胞/骨界面处积累，并且Fc γ。由破骨细胞表达的受体，在同一区域“加盖”，对于这种细胞的起源及其骨附着和再吸收的机制具有重要意义。
Fc receptors, cell surface structures which bind the Fc portion of immunoglobulins, facilitate endocytosis and mediate triggered enzyme release in the phagocytic cells of the mononuclear phagocyte system (MPS). The osteoclast and the macrophage share many similarities, and it has been suggested that they are both derived from a common MPS precursor cell. Nevertheless, Fc.gamma. receptors, found on both primitive and well-differentiated cells of the mononuclear phagocyte system, have yet to be demonstrated on the osteoclast cell membrane. The aim of the present study was first, to study the distribution of rat endogenous IgG around resorbing bone using immunohistochemistry and the avidin-biotin-peroxidase (ABC) technique; and second, to determine if rat osteoclasts express Fc.gamma. receptors, using a recently described technique based on the ABC method. Observations that endogenous IgG accumulated at osteoclast/bone interfaces, and that Fc.gamma. receptors, expressed by osteoclasts, "capped" in the same area, have important implications regarding the origin of this cell and its mechanisms of bone attachment and resorption.