Development of imaging scaffolds for cryo-electron microscopy.
Development of imaging scaffolds for cryo-electron microscopy.
复制标题
DOI：
10.1016/j.sbi.2020.01.012
复制
发表时间：
2020-02
影响因子：
6.8
通讯作者：
T. Yeates;Matthew P Agdanowski;Yuxi Liu
中科院分区：
文献类型：
--
作者：
T. Yeates;Matthew P Agdanowski;Yuxi Liu
随着最近硬件和软件的发展，单粒子冷冻电子显微镜（cryo-EM）已成为最受欢迎的结构生物学工具之一。许多目标，如病毒，大的蛋白质复合物和寡聚膜蛋白，已被解决到原子分辨率使用单粒子cryo-EM，这依赖于从固有噪声投影图像的粒子位置和方向的准确分配。相同的图像处理程序对于较小的蛋白质来说更具挑战性，因为它们的信噪比较低。因此，尽管大多数细胞蛋白质小于50 kDa，但到目前为止，只有少数有利的情况下才有可能解决接近该尺寸范围的冷冻EM结构。在这里，我们强调了一些挑战和最近的努力，以突破这一较低的尺寸限制，工程大型支架刚性显示多个小蛋白质成像。未来的设计努力。
Following recent hardware and software developments, single particle cryo-electron microscopy (cryo-EM) has become one of the most popular structural biology tools. Many targets, such as viruses, large protein complexes and oligomeric membrane proteins, have been resolved to atomic resolution using single-particle cryo-EM, which relies on the accurate assignment of particle location and orientation from intrinsically noisy projection images. The same image processing procedures are more challenging for smaller proteins due to their lower signal-to-noise ratios. Consequently, though most cellular proteins are less than 50 kDa, so far it has been possible to solve cryo-EM structures near that size range for only a few favorable cases. Here we highlight some of the challenges and recent efforts to break through this lower size limit by engineering large scaffolds to rigidly display multiple small proteins for imaging. Future design efforts are noted.