Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis
Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis
复制标题
中国明对虾抗脂多糖因子同工型（FcALF5）的重组表达及功能分析
DOI：
10.1016/j.dci.2015.06.015
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发表时间：
2015-11-01
影响因子：
2.9
通讯作者：
Xiang, Jianhai
中科院分区：
文献类型：
--
作者：
Yang, Hui;Li, Shihao;Xiang, Jianhai
抗菌肽（AMP）具有作为抗生素替代品的巨大潜力，因为AMP不会导致细菌产生耐药性。抗脂多糖因子（ALF）是 AMP 的一种，存在于甲壳类动物中。在本研究中，我们通过原核表达系统从中国明对虾中产生了 ALP 异构体 (FcALF5) 的重组蛋白 (rFcALF5)。 rFcALF5对不同细菌表现出不同的抗菌活性。除了具有抗菌活性外，与白斑综合症病毒（WSSV）预孵育后还可以抑制该病毒对虾的感染。为了了解rFcALF5影响WSSV感染的抗病毒机制，对WSSV总蛋白与rFcALF5之间的相互作用进行了分析，数据显示rFcALF5与WSSV的包膜蛋白VP24有直接相互作用。 FcALF5 的 LPS 结合域 (LBD) 也显示出与 WSSV 的 VP24 的直接相互作用。因此我们推测FcALF5的抗病毒活性可能是通过其LBD与WSSV的VP24结合来实现的。这些发现为制定水产养殖中虾病控制新策略提供了更多信息。 (C) 2015 Elsevier Ltd. 保留所有权利。
Antimicrobial peptides (AMPs) have a great potential to be used as a substitute for antibiotics since AMPs don't lead to bacteria's drug resistance. Anti-lipopolysaccharide factors (ALFs) are one type of AMPs and exist in crustaceans. In the present study, we produced a recombinant protein (rFcALF5) of an ALP isoform (FcALF5) from Chinese shrimp Fenneropenaeus chinensis through a prokaryotic expression system. The rFcALF5 exhibited varied antibacterial activities against different bacteria. Besides its antibacterial activities, it could also inhibit the infection of white spot syndrome virus (WSSV) to shrimp after preincubation with this virus. In order to learn the antiviral mechanism on how rFcALF5 influences WSSV infection, the interaction between the total proteins of WSSV and rFcALF5 was analyzed and the data showed that rFcALF5 had direct interaction with the envelope protein VP24 of WSSV. The LPS binding domain (LBD) of FcALF5 also showed direct interaction with VP24 of WSSV. Therefore we inferred that the antiviral activity of FcALF5 might be achieved through the binding of its LBD to VP24 of WSSV. These findings provided more information to develop new strategies for the control of shrimp disease in aquaculture. (C) 2015 Elsevier Ltd. All rights reserved.