A preclinical model for laryngeal transplantation: Anatomy and mucosal immunology of the porcine larynx
A preclinical model for laryngeal transplantation: Anatomy and mucosal immunology of the porcine larynx
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DOI：
10.1097/00007890-199912150-00006
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发表时间：
1999-12-15
期刊：
影响因子：
6.2
通讯作者：
Bailey, M
中科院分区：
文献类型：
--
作者：
Gorti, GK;Birchall, MA;Bailey, M
背景将喉移植工作转化为临床实践的一个重要步骤是建立临床前模型。对8头明尼苏达小型猪（12-37周龄）的喉进行了解剖学和粘膜免疫学研究。对会厌、声门上、声门和声门下的快速冷冻活检标本进行常规组织学、免疫组化（CD 45）、单双色免疫荧光（CD 3、MHC-II、CD 45）检测。喉骨架的解剖结构与人类喉的解剖结构大致相似。血供主要通过甲状腺尾侧血管，并有颅喉动脉的辅助。猪喉显然具有高度的免疫活性。在粘膜上皮、管腺泡腺周围和偶尔在粘膜下层中发现结构性白细胞聚集。MHC-II和CD 3细胞主要分布于上皮内。所有细胞密度均以会厌最高，其后逐渐降低，声带最低。猪喉在解剖学和免疫学上与人类喉相似，并且含有高水平的免疫组织。它为喉移植提供了理想的临床前模型。
Background. A major step in translating work on laryngeal transplantation into clinical practice is the establishment of a preclinical model. We have investigated the anatomy and mucosal immunology of the porcine larynx in eight Minnesota Minipigs (12-37 weeks).Methods, Neck dissections were carried out and the vascular tree was mapped. Snap-frozen biopsies from epiglottis, supraglottis, glottis, and subglottis were prepared for conventional histology, immunohistochemistry (CD45), and single and two-color immunofluorescence (CD3, MHC-II, CD45).Results. The anatomy of the laryngeal skeleton was broadly similar to that of the human larynx. The blood supply is predominantly via the caudal thyroid vessels, with assistance from the cranial laryngeal artery. The porcine larynx is clearly highly immunologically active. Structured collections of leukocytes were found in the mucosal epithelium, around tubuloacinar glands, and occasionally in the submucosa. MHC-II and CD 3 cells were predominantly found within the epithelium. The highest densities of all cell types were observed in the epiglottis, tailing off caudally, The lowest densities were seen in the vocal cords.Conclusions. The porcine larynx is both anatomically and immunologically similar to the human larynx and contains a high level of immunological organization. It presents an ideal preclinical model for laryngeal transplantation.