A 72KD TROPHOBLAST GLYCOPROTEIN DEFINED BY A MONOCLONAL-ANTIBODY
A 72KD TROPHOBLAST GLYCOPROTEIN DEFINED BY A MONOCLONAL-ANTIBODY
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DOI：
10.1038/bjc.1988.53
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发表时间：
1988-03-01
影响因子：
8.8
通讯作者：
STERN, PL
中科院分区：
文献类型：
--
作者：
HOLE, N;STERN, PL
一种新的滋养层细胞表面抗原已被定义的单克隆抗体5 T4，提出了免疫后与麦胚凝集素（WGA）纯化的糖蛋白从脱氧胆酸盐（DOC）溶解的人合胞体滋养层质膜（StMPM）。通过对正常器官和胎盘组织切片进行间接免疫过氧化物酶染色以及对代表不同正常和肿瘤细胞类型的多种细胞系进行免疫荧光和放射结合测定来确定抗原的分布。在正常足月胎盘的冰冻切片中，5 T4仅在合体滋养层、绒毛外细胞滋养层和羊膜上皮中强烈表达。5 T4抗原显然不被胎盘的任何母体成分表达，也不在成人肝脏、肺、支气管、心脏、睾丸、卵巢、脑或肌肉中检测到。抗原显然由几种特化上皮细胞表达。放射性标记的StMPM的免疫沉淀表明，5 T4分子是mol.重量约为SDS-PAGE分析分子量为72 kD。5 T4抗原由不同的肿瘤细胞系选择性表达，包括发育起源的那些。该抗原的分子特征、相对受限的正常组织分布和某些肿瘤细胞类型的表达使得该抗原值得将来研究用作恶性肿瘤的诊断标志物。
A novel trophoblast cell surface antigen has been defined by a monoclonal antibody 5T4, raised following immunisation with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilized human syncytiotrophoblast plasma membrane (StMPM). The distribution of the antigen was determined by indirect immunoperoxidase staining of sections of normal organ and placental tissues as well as immunofluorescence and radiobinding assays with a wide variety of cell lines representing differing normal and tumour cell types. In frozen sections of normal full term placenta, 5T4 is strongly expressed only by the syncytiotrophoblast, some extravillous cytotrophoblast and the amniotic epithelium. The 5T4 antigen is apparently not expressed by any maternal component of the placenta nor is it detected in adult liver, lung, bronchus, heart, testis, ovary, brain, or muscle. The antigen is apparently expressed by several specialised epithelia. Immunoprecipitation of radiolabelled StMPM indicated that 5T4 molecules are glycoproteins of mol. wt of .apprx. 72 kD on SDS-PAGE. 5T4 antigen is selectively expressed by diverse tumour cell lines, including those of developmental origin. The molecular characteristics, relatively restricted normal tissue distribution and expression by certain tumour cell types makes this antigen worthy of future study for use as a diagnostic marker of malignancy.