Whole-Genome Sequencing and Intraspecific Analysis of the Yeast Species Lachancea quebecensis.
Whole-Genome Sequencing and Intraspecific Analysis of the Yeast Species Lachancea quebecensis.
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DOI：
10.1093/gbe/evv262
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发表时间：
2016-01-05
影响因子：
3.3
通讯作者：
Schacherer J
中科院分区：
文献类型：
--
作者：
Freel KC;Friedrich A;Sarilar V;Devillers H;Neuvéglise C;Schacherer J
酵母群体基因组学的金标准一直是模式生物酿酒酵母。然而，酵母属以外的酵母物种的探索是必不可少的，以扩大对基因组进化的理解。在这里，我们报告的全基因组序列的分析，从最近描述的酵母物种拉茜斯魁北克。对其中一个分离物的基因组进行了组装和注释，并对L.通过将来自8个其它分离物的序列与该参考序列进行比较来调查魁北克分离物。我们的研究表明，这些菌株的基因组平均核苷酸多样性为π = 2 × 10−3，略低于先前确定的S。酿酒酵母（π = 4 × 10−3）。我们的研究结果表明，即使这些分离物都是从一个相对孤立的地理位置，相同的生态来源，并代表一个较小的样本量比S。在酿酒酵母中，分歧水平与在该模式物种中观察到的相似。这种差异主要与存在两个按地理位置划分的不同群组有关。然而，即使基因组分歧的范围相对相似，L。quebecensis具有极低的全球表型方差0.062，而之前在S.啤酒。
The gold standard in yeast population genomics has been the model organism Saccharomyces cerevisiae. However, the exploration of yeast species outside the Saccharomyces genus is essential to broaden the understanding of genome evolution. Here, we report the analyses of whole-genome sequences of nineisolates from the recently described yeast species Lachancea quebecensis. The genome of one isolate was assembled and annotated, and the intraspecific variability within L. quebecensis was surveyed by comparing the sequences from the eight other isolates to this reference sequence. Our study revealed that these strains harbor genomes with an average nucleotide diversity of π = 2 × 10−3 which is slightly lower, although on the same order of magnitude, as that previously determined for S. cerevisiae (π = 4 × 10−3). Our results show that even though these isolates were all obtained from a relatively isolated geographic location, the same ecological source, and represent a smaller sample size than is available for S. cerevisiae, the levels of divergence are similar to those observed in this model species. This divergence is essentially linked to the presence of two distinct clusters delineated according to geographic location. However, even with relatively similar ranges of genome divergence, L. quebecensis has an extremely low global phenotypic variance of 0.062 compared with 0.59 previously determined in S. cerevisiae.