Grape seed proanthocyanidin extract protects against perfluorooctanoic acid-induced hepatotoxicity by attenuating inflammatory response, oxidative stress and apoptosis in mice
Grape seed proanthocyanidin extract protects against perfluorooctanoic acid-induced hepatotoxicity by attenuating inflammatory response, oxidative stress and apoptosis in mice
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葡萄籽原花青素提取物通过减轻小鼠炎症反应、氧化应激和细胞凋亡来防止全氟辛酸诱导的肝毒性
DOI：
10.1039/c5tx00260e
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Zhang, Dalei
中科院分区：
文献类型：
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作者：
Liu, Wenwen;Xu, Changshui;Zhang, Dalei
葡萄籽原花青素提取物（GSPE）是一种丰富的原花青素资源，具有多种生物活性和潜在的健康益处。本研究探讨了GSPE对全氟辛酸（PFOA）引起的小鼠肝损伤的保护作用及其可能的作用机制。连续14天同时用GSPE处理减弱了暴露于PFOA的小鼠肝脏的功能和形态变化。此外，同时补充GSPE减少了炎症细胞因子IL-6和TNF-α的产生，增加了Nrf 2及其靶抗氧化基因超氧化物歧化酶和过氧化氢酶的表达，并减少了暴露于PFOA的小鼠肝脏中丙二醛和过氧化氢的产生。此外，GSPE补充上调抗凋亡蛋白Bcl-2的表达，下调促凋亡蛋白p53和Bax的表达，在PFOA处理的小鼠肝脏中caspase-3的活性降低。这些结果表明，GSPE改善PFOA诱导的小鼠肝脏炎症反应，氧化应激和细胞凋亡。
Grape seed proanthocyanidin extract (GSPE) is a rich source of proanthocyanidins with multiple biological activities and potential health benefits. In the present study, we investigated the protective effect of GSPE against liver injury caused by perfluorooctanoic acid (PFOA) in mice and its possible mechanisms of action. Simultaneous treatment with GSPE for 14 consecutive days attenuated the functional and morphological changes in the liver of PFOA-exposed mice. Furthermore, simultaneous supplementation of GSPE reduced the production of inflammatory cytokines IL-6 and TNF-alpha, increased the expression of Nrf2 and its target antioxidant genes superoxide dismutase and catalase, and decreased the production of malondialdehyde and hydrogen peroxide in the liver of mice exposed to PFOA. Moreover, GSPE supplementation up-regulated the expression of anti-apoptotic protein Bcl-2 and down-regulated the expression of pro-apoptotic proteins p53 and Bax, with a decreased activity of caspase-3 in the liver of PFOA-treated mice. These findings suggest that GSPE ameliorates PFOA-induced inflammatory response, oxidative stress and apoptosis in the liver of mice.