Immunohistochemical and Immunocytochemical Analyses in Patients with Vitreoretinal Lymphoma
Immunohistochemical and Immunocytochemical Analyses in Patients with Vitreoretinal Lymphoma
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DOI：
10.1080/09273948.2018.1533984
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发表时间：
2020-01-02
影响因子：
3.3
通讯作者：
Ishida, Susumu
中科院分区：
文献类型：
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作者：
Kase, Satoru;Namba, Kenichi;Ishida, Susumu
目的：本研究的目的是通过检查玻璃体视网膜淋巴瘤（VRL）患者的眼球摘除和玻璃体细胞块（CB），分析免疫组织化学和免疫细胞学结果。方法：组织学标本来自两个与新生血管性青光眼相关的VRL摘除眼。CB标本制备18例稀释废液含有切碎的玻璃体。提交组织学和细胞学标本进行苏木精-伊红染色和免疫病理学分析。结果：两例标本均可见大量淋巴瘤细胞浸润。摘除眼淋巴瘤细胞CD 20和MUM-1阳性。在VRL的CB淋巴瘤细胞中观察到CD 20的膜免疫反应性。Bcl-6和MUM-1分别在9例检查中的5例和8例中被标记，结论：CB标本的细胞学结果表明类似的组织病理学特征的眼球摘除。从玻璃体废物稀释液中获得的CB标本可作为VRL细胞学诊断的有效材料。
Purpose: The aim of this study was to analyze immunohistochemical and immunocytological findings by examining enucleated eyes and vitreous cell block (CB) in patients with vitreoretinal lymphoma (VRL). Methods: Histological specimens were obtained from two enucleated eyes with VRL associated with neovascular glaucoma. CB specimens were prepared in 18 patients from diluted waste fluids containing shredded vitreous. Histological and cytological specimens were submitted for hematoxylin-eosin staining and immunopathological analyses. Results: Both specimens demonstrated massive infiltration of large lymphoma cells. The lymphoma cells were positive for CD20 and MUM-1 in enucleated eyes. Membranous immunoreactivity for CD20 was observed in lymphoma cells in CB with VRL. Bcl-6 and MUM-1 were marked in five and eight out of nine cases examined, respectively Conclusions: Cytological findings in CB specimens indicated similar histopathological characteristics of enucleated eyes. CB specimens obtained from vitreous waste diluted fluids may serve as effective materials for cytological diagnosis of VRL.