The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner
The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner
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DOI：
10.1016/j.plantsci.2015.10.006
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发表时间：
2015-12-01
期刊：
影响因子：
5.2
通讯作者：
Kim, Ju-Kon
中科院分区：
文献类型：
--
作者：
Lee, Dong-Keun;Kim, Hyung Il;Kim, Ju-Kon
植物对干旱胁迫的响应和适应机制需要通过诱导干旱响应转录因子来调节转录网络。核因子 Y (NF-Y) 转录因子引起了人们对植物干旱胁迫反应作用的兴趣。然而，NF-Y诱导耐旱的分子机制尚不清楚。在这里，我们对两个水稻 NF-YA 基因 OsNF-YA7 和 OsNF-YA4 进行了功能分析。 OsNF-YA7 的表达是由干旱胁迫诱导的，其在转基因水稻植物中的过度表达提高了其耐旱性。相比之下，OsNF-YA4 的表达并未因干旱胁迫而增加，并且其在转基因水稻植物中的过度表达不会影响其对干旱胁迫的敏感性。 OsNF-YA4 的表达受到胁迫相关激素脱落酸 (ABA) 的高度诱导，而 OsNF-YA7 则不然，表明 OsNF-YA7 以不依赖 ABA 的方式介导耐旱性。对 OsNF-YA7 启动子的分析揭示了三个不依赖于 ABA 的 DRE/CTR 元件，RNA-seq 分析确定了 OsNFYA7 作用下游的 48 个基因，这些基因可能参与 OsNF-YA7 介导的耐旱途径。综上所述，我们的结果表明 OsNF-YA7 在水稻干旱胁迫耐受性中发挥着重要作用。 (C) 2015 Elsevier Ireland Ltd. 保留所有权利。
The mechanisms of plant response and adaptation to drought stress require the regulation of transcriptional networks via the induction of drought-responsive transcription factors. Nuclear Factor Y (NF-Y) transcription factors have aroused interest in roles of plant drought stress responses. However, the molecular mechanism of the NF-Y-induced drought tolerance is not well understood. Here, we functionally analyzed two rice NF-YA genes, OsNF-YA7 and OsNF-YA4. Expression of OsNF-YA7 was induced by drought stress and its overexpression in transgenic rice plants improved their drought tolerance. In contrast, OsNF-YA4 expression was not increased by drought stress and its overexpression in transgenic rice plants did not affect their sensitivity to drought stress. OsNF-YA4 expression was highly induced by the stress-related hormone abscisic acid (ABA), while OsNF-YA7 was not, indicating that OsNF-YA7 mediates drought tolerance in an ABA-independent manner. Analysis of the OsNF-YA7 promoter revealed three ABA-independent DRE/CTR elements and RNA-seq analysis identified 48 genes downstream of OsNFYA7 action putatively involved in the OsNF-YA7-mediated drought tolerance pathway. Taken together, our results suggest an important role for OsNF-YA7 in rice drought stress tolerance. (C) 2015 Elsevier Ireland Ltd. All rights reserved.