Muscle Satellite Cell Protein Teneurin-4 Regulates Differentiation During Muscle Regeneration.
Muscle Satellite Cell Protein Teneurin-4 Regulates Differentiation During Muscle Regeneration.
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DOI：
10.1002/stem.2058
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Akazawa C
中科院分区：
文献类型：
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作者：
Ishii K;Suzuki N;Mabuchi Y;Ito N;Kikura N;Fukada S;Okano H;Takeda S;Akazawa C
卫星细胞维持在未分化的静止状态，但在肌肉再生过程中，它们进入激活阶段，开始增殖并分化为成肌细胞。跨膜蛋白teneurin‐4 （Ten‐4）在静止卫星细胞中特异性表达；然而，其细胞和分子功能尚不清楚。因此，我们旨在阐明Ten‐4在肌肉卫星细胞中的功能。在Ten‐4‐缺陷小鼠的胫骨前肌（TA）中，无论是否诱导肌肉再生，肌纤维的数量和大小以及卫星细胞的数量都减少了。此外，我们发现再生的Ten‐4‐缺陷TA肌肉中卫星细胞的激活加速。用原代卫星细胞进行的细胞培养分析表明，Ten‐4抑制了肌源性分化的进展。总之，我们的研究结果表明，Ten‐4在维持肌肉卫星细胞的静止中起着至关重要的作用。干细胞2015；33:3017 - 3027
Satellite cells are maintained in an undifferentiated quiescent state, but during muscle regeneration they acquire an activated stage, and initiate to proliferate and differentiate as myoblasts. The transmembrane protein teneurin‐4 (Ten‐4) is specifically expressed in the quiescent satellite cells; however, its cellular and molecular functions remain unknown. We therefore aimed to elucidate the function of Ten‐4 in muscle satellite cells. In the tibialis anterior (TA) muscle of Ten‐4‐deficient mice, the number and the size of myofibers, as well as the population of satellite cells, were reduced with/without induction of muscle regeneration. Furthermore, we found an accelerated activation of satellite cells in the regenerated Ten‐4‐deficient TA muscle. The cell culture analysis using primary satellite cells showed that Ten‐4 suppressed the progression of myogenic differentiation. Together, our findings revealed that Ten‐4 functions as a crucial player in maintaining the quiescence of muscle satellite cells. Stem Cells 2015;33:3017–3027