In vivo label-free confocal imaging of adult mouse brain up to 1.3-mm depth with NIR-II illumination
In vivo label-free confocal imaging of adult mouse brain up to 1.3-mm depth with NIR-II illumination
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DOI：
10.1117/12.2509132
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Fei Xia;Chunyan Wu;D. Sinefeld;Bo Li;Yifan Qin;Chris Xu
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文献类型：
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作者：
Fei Xia;Chunyan Wu;D. Sinefeld;Bo Li;Yifan Qin;Chris Xu
我们将1.7mNIR-II照射与反射式共聚焦显微镜相结合，在成年小鼠脑内获得了~1.3 mm的高空间分辨率成像深度，是传统可见光共聚焦显微镜成像深度的3~4倍。我们证明，该方法可以作为任何具有低成本光源和探测器的激光扫描显微镜的附加通道，例如连续波(CW)二极管激光器和InGaAs光电二极管。该技术无需标记，简单，需要低照明功率，有可能为各种生物和临床应用中的深层组织成像创造新的机会。
We combined NIR-II illumination at ~1.7 μm with reflectance confocal microscopy and achieved an imaging depth of ~1.3 mm with high spatial resolution in adult mouse brain in vivo, which is 3-4 times deeper than that of conventional confocal microscopy using visible wavelength. We showed that the method can be added as an additional channel to any laser-scanning microscope with low-cost sources and detectors, such as continuous-wave (CW) diode lasers and InGaAs photodiodes. The technique is label-free, simple and requires low illumination power, potentially creating new opportunities for deep tissue imaging in various biological and clinical applications.