Application of AFM to the Nanomechanics of Cancer
Application of AFM to the Nanomechanics of Cancer
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DOI：
10.1557/adv.2016.255
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发表时间：
2016-01-01
期刊：
影响因子：
0.8
通讯作者：
Gimzewski, James K.
中科院分区：
文献类型：
--
作者：
Sharma, Shivani;Gimzewski, James K.
癌细胞转移是死亡的主要原因，癌细胞从肿瘤迁移并扩散到体内的远处部位。了解转移需要在细胞水平上更深入地了解生物力学和机械生物学。我们已经建立了使用原子力显微镜通过测量杨氏模量来推断培养物中单细胞的机械特性。在这里，我们讨论基于 AFM 的细胞力学研究的主要优势、挑战、技术限制和适用性，以及其他新兴的高通量技术，用于开发基于单细胞力学的癌症检测和管理临床分析。
Cancer cell metastasis is a leading cause of mortality whereby cancer cells migrate from a tumor and spread to distant sites in the body. Understanding metastasis requires a deeper understanding of biomechanics and mechanobiology at the cellular level. We have established the use of Atomic Force Microscopy to infer the mechanical properties of single cells in cultures by measurement of their Young's modulus. Here we discuss the main advantages, challenges, technological limitations and applicability of AFM based cell mechanics studies along with other emerging high throughput techniques for the development of single cell mechanical based clinical assays for cancer detection and management.