Apoptosis and cell cycle arrest in oral lichen planus hypothesis on their possible influence on its malignant transformation
Apoptosis and cell cycle arrest in oral lichen planus hypothesis on their possible influence on its malignant transformation
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DOI：
10.1016/j.archoralbio.2005.02.005
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发表时间：
2005-10-01
影响因子：
3
通讯作者：
Bascones, A
中科院分区：
文献类型：
--
作者：
Bascones, C;Gonzalez-Moles, MA;Bascones, A
分析口腔扁平苔藓(OLP)中细胞周期停滞和细胞凋亡机制的定量重要性，以评估细胞对T淋巴细胞攻击的反应，并就这些现象在口腔扁平苔藓恶变过程中的影响建立假说。采用原位末端标记法和免疫组织化学方法检测32例口腔扁平苔藓和20例正常口腔黏膜组织中caspase-3、Bax和p21的表达。TUNEL、caspase-3和p21的阳性率在病例组显著高于对照组(p<；0.001)。TUNEL和caspase-3在基底层的表达均显著高于基底层(p分别为0.004和0.052)。液化程度越重，p21的基础和超基础表达水平越高(p<；0.01)。Bax的基础表达在病例组和对照组之间无显著差异。在口腔扁平苔藓中，细胞凋亡的数量重要性很小。口腔扁平苔藓中的上皮细胞对细胞凋亡和细胞周期停滞的反应非常低，这可能会产生一种有利于恶性转化的上皮底物。(C)2005爱思唯尔有限公司。保留所有权利。
The quantitative importance of cell cycle arrest and apoptosis mechanisms in oral Lichen planus (OLP) was analysed in order to assess the cell response to T lymphocyte aggression and establish a hypothesis on the influence of these phenomena in the malignant transformation process. The TUNEL assay and immunohistochemical methods were used to detect caspase-3, bax, and p21 in 32 tissue samples of oral mucosa with OLP and in 20 samples of normal oral mucosa. Positivity for TUNEL, caspase-3 and p21 was significantly more frequent in cases than in controls (p < 0.001). Both TUNEL and caspase-3 positivity was significantly greater in the basal versus suprabasal layer (p = 0.004 and 0.052, respectively). The basal and suprabasal expression of p21 was significantly higher in cases with a more intense liquefaction degeneration (p < 0.01). There was no significant difference in basal expression of bax between cases and controls. The quantitative importance of apoptosis was small in OLP. Epithelial cells attacked in OLP have a very low response to apoptosis and cell cycle arrest mechanisms, which may produce an epithelial substrate that favours malignant transformation. (c) 2005 Elsevier Ltd. ALL rights reserved.