Contact Mapping to Unravel Chromosome Folding.
Contact Mapping to Unravel Chromosome Folding.
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接触图谱揭示染色体折叠。
DOI：
10.1016/j.tibs.2019.09.006
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发表时间：
2019
影响因子：
13.8
通讯作者：
Eagen,KyleP
中科院分区：
文献类型：
--
作者：
Ge,Tiffany;Rosencrance,CelesteD;Eagen,KyleP
由于观察DNA折叠的方法不足，核结构仍然是神秘的。新技术旨在通过绘制染色质接触图来探测染色体组织。染色体构象捕获（3C）最初依赖于染色质的化学交联，然后通过邻近连接来恢复相邻基因组位点的空间信息。基于连接的方法已经从测量成对接触（Hi-C）发展到少数（COLA、Tri-C、MC-4C）和几十个（C-walk）全基因组范围的相互作用。无连接的方法增强了不同的染色体区域之间的高阶相互作用的识别，以及那些在核隔室。这些新方法提供了来自低细胞输入的无偏空间信息（基因组结构作图[GAM]），鉴定了多个基因组位点之间的同时相互作用（通过标签延伸的相互作用的分裂池识别[SPRITE]），并确定了单分子水平的精确相互作用（ChIA-Drop）。
Nuclear architecture has remained mysterious due to inadequate methods for observing DNA folding. New technologies aim to probe chromosome organization by mapping chromatin contacts. Chromosome conformation capture (3C) originally relied on chemical crosslinking of chromatin followed by proximity ligation to recover spatial information of neighboring genomic loci. Ligation-based methods have progressed from measuring pairwise contacts (Hi-C), to a handful (COLA, Tri-C, MC-4C) and dozens (C-walks) of interactions genome-wide.Technologies based on proximity ligation inefficiently detect multiple interactions per locus. Ligation-free methods enhance the identification of higher-order interactions among distinct chromosomal regions as well as those within nuclear compartments. These novel methods provide unbiased spatial information from low cell inputs (Genome Architecture Mapping [GAM]), identify simultaneous interactions between multiple genomic loci (Split-pool Recognition of Interactions by Tag Extension [SPRITE]), and determine precise interactions at the single molecule level (ChIA-Drop).