Plasmid-controlled mercury biotransformation by Clostridium cochlearium T-2
Plasmid-controlled mercury biotransformation by Clostridium cochlearium T-2
复制标题
Clostridium cochlearium T-2 的质粒控制汞生物转化
DOI：
10.1128/aem.40.6.1007-1011.1980
复制
发表时间：
1980
影响因子：
4.4
通讯作者：
N. Imura
中科院分区：
文献类型：
--
作者：
H. Pan;M. Hosono;N. Imura
分离到一株具有降解甲基汞能力的耳蜗梭菌。该能力通过吖啶染料处理而治愈，并通过治愈的菌株与亲本菌株的接合而恢复。然后，如先前报道的，固化的菌株显示出甲基化汞离子的活性（M.山田和K. Tonomura，J. Ferment. 50：159-166，1971）。这些结果和琼脂糖凝胶电泳模式的脱氧核糖核酸的裂解物表明可能的作用，质粒在控制汞的生物转化的两个相反的方向在一个单一的细菌菌株：甲基化的情况下的质粒和去甲基化的存在下it.A可能的机制，涉及硫化氢的耐汞性进行了讨论。
A strain of Clostridium cochlearium having methylmercury-decomposing ability was isolated. The ability was cured by the treatment with acridine dye and recovered by the conjugation of the cured strain with the parent strain. The cured strain then showed the activity to methylate mercuric ion as previously reported (M. Yamada and K. Tonomura, J. Ferment. Technol. 50:159-166, 1971). These results and the agarose gel electrophoretic pattern of the deoxyribonucleic acids from the lysates indicate a possible role of plasmids in controlling the mercury biotransformation of the two opposite directions in a single bacterial strain: methylation in the absence of the plasmid and demethylation in the presence of it. A possible mechanism for mercury resistance involving hydrogen sulfide is discussed.