Six6 (Optx2) is a novel murine Six3-related homeobox gene that demarcates the presumptive pituitary/hypothalamic axis and the ventral optic stalk
Six6 (Optx2) is a novel murine Six3-related homeobox gene that demarcates the presumptive pituitary/hypothalamic axis and the ventral optic stalk
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DOI：
10.1016/s0925-4773(99)00068-4
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发表时间：
1999-06-01
影响因子：
2.6
通讯作者：
Gruss, P
中科院分区：
文献类型：
--
作者：
Jean, D;Bernier, G;Gruss, P
我们报道了一个含有小鼠同源盒的基因Six6(Optx2)的分离，该基因在其编码区与Six3有广泛的同源性，Six3是哺乳动物Six基因家族中唯一已知在视神经原基中表达的成员。系统发育分析表明，Six6和Six3属于一组独立的同源盒基因-ART：与最近发现的果蝇Optix密切相关。最早的Six6表达在原始前脑的间脑部分的Boor处被检测到，该区域被预测为产生神经垂体和下丘脑的区域。后来，在原始组织中发现了Six6mRNA，这些原始组织形成了成熟的垂体腺：Rathke氏囊和漏斗隐窝。在视神经原基中，Six6划分了假定的腹侧视柄和未来神经视网膜的腹侧部分。在发育期，Six6在神经视网膜、视交叉和视柄中有表达，但在晶状体中没有表达。与Six6相比，SID的表达模式非常相似，但转录本在大脑和视觉系统中的分布普遍更广泛。我们最终发现Six6不需要Pax6在视原基中表达，这表明在控制眼睛早期发育的遗传级联中，Six6与Pax6在平行和/或独立的途径上起作用。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
We report on the isolation of a murine homeobox-containing gene, Six6 (Optx2), that shows extended identity in its coding region with Six3, the only member of the mammalian Six gene family known to be expressed in the optic primordium. Phylogenetic analysis demonstrates that Six6 and Six3 belong to a separate group of homeobox-genes that art: closely related to the recently identified Drosophila optix. Earliest Six6 expression was detected in the Boor of the diencephalic portion of the primitive forebrain, a region predicted to give rise to the neurohypophysis and to the hypothalamus. Later on, Six6 mRNA was found in the primordial tissues giving rise to the mature pituitary: the Rathke's pouch and the infundibular recess. In the optic primordium, Six6 demarcates the presumptive ventral optic stalk and the ventral portion of the future neural retina. In the developing eye, Six6 expression was detected in the neural retina, the optic chiasma and optic stalk, but nut in the lens. When compared to Six6, Sid expression pattern was highly similar, but with a generally broader transcripts distribution in the brain and in the visual system. We finally show that Six6 does not require Pax6 for its expression in the optic primordium, suggesting that Six6 acts on a parallel and/or independent pathway with Pax6 in the genetic cascade governing early development of the eye. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.