Cycloheximide induces synchronous swelling of perialgal vacuoles enclosing symbiotic Chlorella vulgaris and digestion of the algae in the ciliate Paramecium bursaria
Cycloheximide induces synchronous swelling of perialgal vacuoles enclosing symbiotic Chlorella vulgaris and digestion of the algae in the ciliate Paramecium bursaria
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DOI：
10.1016/j.protis.2008.02.005
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发表时间：
2008-07-07
期刊：
影响因子：
2.5
通讯作者：
Fujishima, Masahiro
中科院分区：
文献类型：
--
作者：
Kodama, Yuuki;Fujishima, Masahiro
放线菌酮对草履虫共生小球藻的蛋白质合成有明显的抑制作用，但对宿主蛋白质合成几乎没有影响。用放线菌酮处理草履虫细胞，会引起所有位于宿主细胞膜下方的藻周空泡同步肿胀。在这项研究中，共生藻细胞壁和藻周液泡膜之间的空间扩大到约25倍，其正常宽度24小时后，放线菌酮处理。然后，将从宿主细胞膜下分离的空泡浓缩并用Gomori溶液染色，并消化空泡中的藻类。尽管这种现象仅在荧光条件下诱导，而在恒定黑暗条件下不会诱导，但在光合作用抑制剂3-（3，4-二氯苯基）-1，1-二甲基脲存在的情况下，在光照下用环己酰亚胺处理的草履虫中不会诱导这种现象。这些结果表明，在藻类光合作用的存在下合成的藻类蛋白质服务于一些重要的功能，以防止扩大的藻周液泡和保持藻周液泡膜的能力，以保护自己免受宿主溶酶体融合。(c)2008爱思唯尔有限公司All rights reserved.
Cycloheximide is known to inhibit preferentially protein synthesis of symbiotic Chlorella of the ciliate Paramecium bursaria, but to hardly host protein synthesis. Treatment of algae-bearing Paramecium cells with cycloheximide induces synchronous swelling of all perialgal vacuoles that are localized immediately beneath the host's cell membrane. In this study, the space between the symbiotic algal cell wall and the perialgal vacuole membrane widened to about 25 times its normal width 24 h after treatment with cycloheximide. Then, the vacuoles detached from beneath the host's cell membrane, were condensed and stained with Gomori's solution, and the algae in the vacuoles were digested. Although this phenomenon is induced only under a fluorescent light condition, and not under a constant dark condition, this phenomenon was not induced in paramecia treated with cycloheximide in the light in the presence of the photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. These results indicate that algal proteins synthesized in the presence of algal photosynthesis serve some important function to prevent expansion of the perialgal vacuole and to maintain the ability of the perialgal vacuole membrane to protect itself from host lysosomal fusion. (c) 2008 Elsevier GmbH. All rights reserved.