Growth, physiological responses, and copper accumulation in seven willow species exposed to Cu-a hydroponic experiment
Growth, physiological responses, and copper accumulation in seven willow species exposed to Cu-a hydroponic experiment
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DOI：
10.1007/s11356-018-2106-z
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发表时间：
2018-07-01
影响因子：
5.8
通讯作者：
Chen, Guangcai
中科院分区：
文献类型：
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作者：
Cao, Yini;Zhang, Ying;Chen, Guangcai
选择能够耐受和积累高水平重金属的植物，特别是木本植物，是植物修复的关键。本研究以7个柳树品种/无性系为研究对象，在不同剂量（对照、15和120 μ M）的水培条件下，对铜（Cu）的耐受性、Cu积累和相对生理反应进行了研究。在铜暴露后，所有被测试的柳树种类/无性系（Salix， S.）保持相对正常的生长，尽管观察到一些Cu毒性的视觉证据。在120亩铜胁迫下，7种柳树的总生物量相对较高，耐受性指数为>.6，表明其具有较高的耐铜性。120亩M Cu处理后，所有柳树的光合作用均显著下降（16.3 ~ 76.1%）。120亩M Cu处理后，5种柳（整柳“一芝壁”、江水柳“J172”、松14、松25、松89）叶片可溶性糖含量升高，可溶性蛋白含量降低。铜主要富集在根系，含量在1916 ~ 26,244 mg kg(-1) DW之间。主成分分析和隶属函数分析表明，松茸89和松茸25的生物量和积累能力明显高于其他树种。这表明这两个柳树无性系可以作为污染土壤中Cu的植物稳定的潜在候选者。
Selecting plant species, especially woody species, that can tolerate and accumulate high levels of heavy metals is crucial for the purpose of phytoremediation. In the present study, seven willow species/clones were evaluated for their variations in copper (Cu) tolerance, Cu accumulation, and their relative physiological responses, when exposed to different doses of Cu (control, 15, and 120 mu M) in a hydroponic system for 40 days. Upon Cu exposure, all tested willow species/clones (Salix, S.) remained relative normal growth, albeit with some visual evidence of Cu toxicity observed. Seven willow species remained relative high total biomass with tolerance index > 0.6 when being exposed to 120 mu M Cu, suggesting their high Cu tolerance. Exposure to 120 mu M Cu resulted in notable declines (16.3-76.1%) in photosynthesis in all willow species. Increases in the soluble sugar content and decreases in the soluble protein content in the leaves of five willow species (S. integra "Yizhibi", S. jiangsuensis "J172", S. matsudana 14, S. matsudana 25, S. matsudana 89) were found in the 120 mu M Cu treatment. The majority of Cu mainly accumulated in the roots, ranging from 1916 to 26,244 mg kg(-1) DW. Principal component analysis and membership function analysis suggested that S. matsudana 89 and S. matsudana 25 showed much higher biomass and accumulation ability than the other species. This suggests that these two willow clones could be used as potential candidates for the phytostabilization of Cu in contaminated soils.