XPNPEP2 is associated with lymph node metastasis in prostate cancer patients
XPNPEP2 is associated with lymph node metastasis in prostate cancer patients
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XPNPEP2与前列腺癌患者的淋巴结转移相关
DOI：
10.1038/s41598-019-45245-5
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发表时间：
2019-07-11
影响因子：
4.6
通讯作者：
Ma，Xiangyi
中科院分区：
文献类型：
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作者：
Li，Fei;Dai，Yun;Ma，Xiangyi
正如我们在以前的研究中所报道的，TMTP 1是一种肿瘤归巢肽，选择性地靶向高转移性肿瘤及其转移灶。氨肽酶P2（XPNPEP 2）是TMTP 1肿瘤归巢肽的受体。然而，氨肽酶P2在人类癌症中的生物学和临床意义仍然未知。在这项研究中，高密度多器官肿瘤组织阵列用于分析XPNPEP 2在人类标本中的表达谱。结果显示，XPNPEP 2在正常前列腺组织中呈中等表达，而在前列腺癌中表达显著降低。因此，我们使用TCGA、IHC和ELISA进一步分析XPNPEP 2在前列腺癌患者组织和血清中的表达。一般而言，XPNPEP 2在前列腺癌组织中的表达低于正常前列腺组织，但在具有局部浸润和LN转移的前列腺癌组织中的表达高于具有局部Pca的组织。Western blot证实XPNPEP 2在血清中以分泌形式存在。128例Pca患者血清，检测70例健康男性和40例前列腺增生患者血清中XPNPEP 2的含量，结果表明：前列腺增生伴LN转移患者血清中XPNPEP 2的含量明显高于正常对照组（P < 0.01），Pca患者血清TNF-α水平（142.7 ± 14.40 ng/mL）明显高于无淋巴结转移的Pca患者（61.63 ± 5.50 ng/mL）（P< 0.01）。ROC曲线分析显示PSA和XPNPEP 2联合检测对LN转移的预测效果优于PSA或XPNPEP 2单独检测，尤其是对PSA水平低的Pca患者。总之，血清XPNPEP 2水平与PSA水平结合可能导致预测Pca患者LN转移的敏感性增加，特别是对于血清PSA水平低的患者。
As we reported in our previous studies, TMTP1, a tumor-homing peptide, selectively targets highly metastatic tumors and their metastatic foci. Aminopeptidase P2 (XPNPEP2) is a receptor for TMTP1 tumor-homing peptide. However, the biological and clinical significance of Aminopeptidase P2 in human cancers remains unknown. In this study, the high-density multiple organ tumor tissue array was employed for the analysis of XPNPEP2 expression profiles in human specimens. The results showed that XPNPEP2 was moderately expressed in the normal prostate tissues, but significantly decreased in the prostate cancer. Hence we used TCGA, IHC, and ELISA to further analyze the expression of XPNPEP2 in tissues and serum of prostate cancer patients. In general, XPNPEP2 expression was lower in prostate cancer tissue than in normal prostate tissue, but was higher in prostate cancer tissues with local invasion and LN metastasis than in tissues with localized Pca. Western blot clarified XPNPEP2 had a secreted form in the serum. Then the serums of 128 Pca patients, 70 healthy males and 40 prostate hyperplasia patients were obtained for detecting serum XPNPEP2 levels.The results indicated that the concentration of XPNPEP2 in serums of Pca patients with LN metastasis (142.7 ± 14.40 ng/mL) were significantly higher than levels in Pca patients without LN metastasis (61.63 ± 5.50 ng/mL) (p< 0.01). An ROC analysis revealed that the combination of PSA and XPNPEP2 was more efficient than PSA or XPNPEP2 alone for predicting LN metastasis, especially for Pca patients with low serum PSA levels. In summary, serum XPNPEP2 levels when combined with PSA levels may result in increased sensitivity for predicting LN metastasis in Pca patients, especially for patients with low serum PSA levels.