Appearance of functional EGF receptor kinase during rodent embryogenesis.
Appearance of functional EGF receptor kinase during rodent embryogenesis.
复制标题
啮齿动物胚胎发生过程中功能性 EGF 受体激酶的出现。
DOI：
10.1002/j.1460-2075.1983.tb01682.x
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Webb,CG
中科院分区：
文献类型：
--
作者：
Hortsch,M;Schlessinger,J;Gootwine,E;Webb,CG
研究了不同发育阶段小鼠和大鼠胚胎组织的表皮生长因子（EGF）受体激酶活性。胚胎组织磷酸化的EGF受体在SDS凝胶上显示为分子量为17万道尔顿的条带。在妊娠10天以后发育中的小鼠胎儿中就可以清楚地证明这一点。研究了EGF受体激酶在13日龄小鼠胎儿各组织中的分布。这种活动在皮肤、正在发育的骨骼肌和各种内脏中都很明显，但在肝脏和大脑中却明显不存在。羊膜是活性最丰富的来源之一，而卵黄囊呈阴性，胎盘呈弱阳性。16日龄大鼠胎儿的分布与13日龄小鼠胎儿的分布非常相似。肝脏在妊娠18天获得EGF受体激酶活性，并在新生儿中具有较高的活性。磷氨基酸分析表明，磷酪氨酸是胚胎组织中主要的标记氨基酸残基。因此，通过免疫沉淀和磷酸化研究的胎儿组织中的EGF受体似乎与成年哺乳动物组织中的EGF受体相似。这种功能性EGF受体激酶活性可以在器官发生开始时首次检测到。
Mouse and rat embryonic tissues at various stages of development were examined for epidermal growth factor (EGF) receptor kinase activity. The phosphorylated EGF receptor from embryonic tissues appeared as a band of mol. wt. 170 000 daltons on SDS gels. It was clearly demonstrable in the developing mouse fetus from 10 days of gestation onwards. The distribution of the EGF receptor kinase was studied in various tissues of 13 day mouse fetuses. The activity was apparent in the skin, developing skeletal muscles and various internal organs but was notably absent in the liver and brain. The amnion was found to be one of the richest sources of activity while the yolk sac was negative, and the placenta was weakly positive. In 16 day rat fetuses the distribution was quite similar to that of the 13 day mouse fetus. The liver acquired EGF receptor kinase activity by 18 days of gestation and had high activity in neonates. Phosphoamino acid analysis revealed that phosphotyrosine was the major labelled amino acid residue in the embryonic tissues. Thus, the EGF receptor of fetal tissues as studied by immune precipitation and phosphorylation appears to be a similar entity to that found in adult mammalian tissues. This functional EGF receptor kinase activity could first be detected at the time of onset of organogenesis.