Cellular requirements for thyrotropin enhancement of in vitro antibody production.
Cellular requirements for thyrotropin enhancement of in vitro antibody production.
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DOI：
10.4049/jimmunol.137.1.197
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发表时间：
1986-07
影响因子：
4.4
通讯作者：
T. Kruger;J. Blalock
中科院分区：
文献类型：
--
作者：
T. Kruger;J. Blalock
我们以前报道过促甲状腺激素(TSH)增强T细胞依赖抗原绵羊红细胞(SRBC)的体外抗体反应，这是通过直接空斑形成细胞(PFC)试验确定的。本研究旨在探讨促甲状腺激素(TSH)对流产布鲁氏菌非T抗原(BA-TNP)免疫淋巴细胞的可能免疫调节作用及其相关细胞成分。我们在这里报告，TSH增强了对BA-TNP的体外抗体反应，这是通过直接的PFC试验确定的。细胞耗竭研究表明，TSH效应虽然独立于巨噬细胞，但需要T细胞的存在。因此，垂体--可能还有白细胞--TSH似乎是一种淋巴因子，它可能通过T细胞发挥作用，增加抗体的产生。
We reported previously that thyrotropin (TSH) enhanced the in vitro antibody response to a T cell-dependent antigen, sheep red blood cells (SRBC), as determined by direct plaque-forming cell (PFC) assay. The present studies were designed to determine the possible immunoregulatory function of TSH on lymphocytes immunized with the T-independent antigen Brucella abortus-TNP (BA-TNP) and the cellular components involved in such function. We report here that TSH enhanced the in vitro antibody response to BA-TNP as determined by direct PFC assays. Cell depletion studies showed that the TSH effect, although independent of macrophages, required the presence of T cells. Thus, pituitary--and possibly leukocyte--TSH appears to function as a lymphokine which may act via T cells to augment antibody production.