Preparation of high quality nanowires by tobacco mosaic virus templating of gold nanoparticles
Preparation of high quality nanowires by tobacco mosaic virus templating of gold nanoparticles
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DOI：
10.1039/b809585j
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Mann, Stephen
中科院分区：
文献类型：
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作者：
Bromley, Keith M.;Patil, Avinash J.;Mann, Stephen
报道了一种制备定义明确的金属化烟草花叶病毒(TMV)纳米棒的稳定悬浮液的方法，该方法高产率且涂层均匀。通过添加等量的金前驱体(HAuCl4)和还原剂(NaBH4)，在五个加还原循环中递增地控制病毒粒子表面的无机生长，以获得由致密堆积的金纳米颗粒组成的杂化各向异性纳米结构。在协议中引入了几个新的步骤，包括在第一次加减循环后加入乙醇和用聚L赖氨酸包裹TMV/Au纳米杂化材料，以确保纳米线悬浮液的高度均匀和稳定。通过将反应循环的次数限制在四次以下，实现了对金属化纳米结构中颗粒间距的控制。利用激光光散射显微镜对生物介导还原的重要性进行了探讨。结果表明，较短的孵育时间与增加纳米粒子的单分散性和高保真复制是一致的。
A protocol for the preparation of stable suspensions of well-defined metallized tobacco mosaic virus (TMV) nanorods in high yields and with uniform coatings is reported. Inorganic growth on the virion surface was controlled by adding aliquots of gold precursor (HAuCl4) and reducing agent (NaBH4) incrementally over five addition-reduction cycles to produce hybrid anisotropic nanostructures consisting of densely packed gold nanoparticles. Several novel steps, including addition of ethanol after the first addition-reduction cycle and wrapping of the TMV/Au nanohybrids with poly-L-lysine, were introduced into the protocol to ensure high homogeneity and stability in the nanowire suspension. Control of the interparticle spacing in the metallized nanostructures was achieved by restricting the number of reaction cycles used to less than four iterations. The importance of bio-mediated reduction was probed using laser light scattering microscopy. The results indicated that short incubation times were consistent with increased nanoparticle monodispersity and high fidelity replication.