Anomalous, non-Gaussian, viscoelastic, and age-dependent dynamics of histonelike nucleoid-structuring proteins in live Escherichia coli
Anomalous, non-Gaussian, viscoelastic, and age-dependent dynamics of histonelike nucleoid-structuring proteins in live Escherichia coli
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DOI：
10.1103/physreve.98.042411
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发表时间：
2018-10
影响因子：
2.4
通讯作者：
Yong Wang;Asmaa A. Sadoon
中科院分区：
文献类型：
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作者：
Yong Wang;Asmaa A. Sadoon
我们报告我们的测量组蛋白样的类核结构（H-NS）蛋白，同时与蛋白质和DNA相互作用的动态，在活大肠杆菌。动力学结果与以前报道的其他分子显着不同。观察到单个H-NS蛋白的扩散系数呈幂律分布。此外，我们观察到的分布的位移不遵循高斯，柯西，或拉普拉斯分布，但皮尔逊VII型分布。此外，我们实验测量的时间和频率依赖性的细菌细胞质，偏离均匀的蛋白质溶液的粘弹性，并显示玻璃-液体转变的复数模量。最后，我们观察到H-NS蛋白的动态是细胞长度和细胞年龄依赖性的。这些发现有望从根本上改变目前对细菌细胞质和细菌分子扩散动力学的看法。
We report our measurements of the dynamics of histonelike nucleoid-structuring (H-NS) proteins, which interact with both proteins and DNA simultaneously, in liveEscherichia colibacteria. The dynamics turn out to differ significantly from other molecules reported previously. A power-law distribution was observed for the diffusion coefficients of individual H-NS proteins. In addition, we observed a distribution of displacements which does not follow the Gaussian, Cauchy, or Laplace distributions but the Pearson Type VII distribution. Furthermore, we experimentally measured the time and frequency dependence of the complex modulus of the bacterial cytoplasm, which deviates from the viscoelasticity of homogeneous protein solutions and shows a glass-liquid transition. Last, we observed that the dynamics of H-NS proteins is cell length and cell age dependent. The findings are expected to fundamentally change the current views on bacterial cytoplasm and diffusional dynamics of molecules in bacteria.