The histone deacetylase HOS2 controls pathogenicity through regulation of melanin biosynthesis and appressorium formation in Colletotrichum gloeosporioides
The histone deacetylase HOS2 controls pathogenicity through regulation of melanin biosynthesis and appressorium formation in Colletotrichum gloeosporioides
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DOI：
10.1186/s42483-022-00126-0
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发表时间：
2022-06-06
影响因子：
3.4
通讯作者：
An, Bang
中科院分区：
文献类型：
--
作者：
Liu, Shike;Wang, Qiannan;An, Bang
组蛋白的可逆乙酰化可有效控制染色质动力学，并在真核生物中发挥至关重要的作用。在本研究中，我们在胶孢炭疽病菌中鉴定并鉴定了一种组蛋白脱乙酰酶 HOS2 直向同源物，命名为 CgHOS2。敲除 CgHOS2 会导致营养生长减少、分生孢子形成受损和胁迫耐受性降低。此外，Delta CgHOS2突变体无法形成附着细胞，并且失去了对完整植物叶子的致病性。 Western blot 分析表明，CgHOS2 负责组蛋白 H3 的脱乙酰化。通过转录组分析，预测了CgHOS2控制的一系列候选基因。在这些候选基因中，黑色素生物合成相关酶的表达在营养菌丝中特别是在附着胞中显着降低，从而导致黑色素含量减少和附着胞形成失败。总而言之，这些结果强调了 CgHOS2 通过调节胶孢炭疽菌中黑色素生物合成而在致病性中的作用。
The reversible acetylation of histones is effective for controlling chromatin dynamics and plays crucial roles in eukaryotes. In the present study, we identified and characterized a histone deacetylase HOS2 ortholog, designated CgHOS2, in Colletotrichum gloeosporioides. Knocking out CgHOS2 resulted in decreased vegetative growth, impaired conidiation, and reduced stress tolerance. Moreover, the Delta CgHOS2 mutant failed to form appressoria and lost pathogenicity on intact plant leaves. Western blot analysis revealed that CgHOS2 is responsible for the deacetylation of histone H3. Via transcriptomic analysis, a series of candidate genes controlled by CgHOS2 were predicted. Of these candidate genes, the expression of melanin biosynthesis-related enzymes was significantly reduced in vegetative hyphae and especially in appressoria, which led to a decrease in melanin content and failure of appressorium formation. Taken together, these results highlight the role of CgHOS2 in pathogenicity via regulation of melanin biosynthesis in C. gloeosporioides.