Suppressive effects of genistein on oxidative stress and NFκB activation in RAW 264.7 macrophages
Suppressive effects of genistein on oxidative stress and NFκB activation in RAW 264.7 macrophages
复制标题
DOI：
10.1271/bbb.67.1916
复制
发表时间：
2003-09-01
影响因子：
1.6
通讯作者：
Song, Y
中科院分区：
文献类型：
--
作者：
Choi, C;Cho, H;Song, Y
本研究旨在探讨金雀异黄素是否能减轻脂多糖刺激的RAW 264.7小鼠巨噬细胞株的氧化应激和核因子kappaB的活化。金雀异黄素处理RAW 264.7细胞可显著减少脂多糖刺激的一氧化氮生成，且呈剂量依赖关系，IC50值为69.4um。50um和100um浓度的金雀异黄素减少了硫代巴比妥酸反应物质(TBARS)的积累，提高了GSH水平和抗氧化酶活性，如超氧化物歧化酶(SOD)和过氧化氢酶(CAT)。核因子kappaB(NFkappaB)在50 mU和100 mU金雀异黄素处理组的核提取液上的DNA结合活性受到显著抑制。这些结果表明，金雀异黄素具有温和的抗氧化活性，可以抑制细胞内氧化应激和NFkappaB的激活。
This study was designed to investigate whether genistein may ameliorate oxidative stress and nuclear factor kappaB (NFkappaB) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with genistein significantly reduced lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in a dose-dependent manner with an IC50 of 69.4 mum. Genistein at 50 mum and 100 mum concentrations reduced thiobarbituric acid-reactive substances (TBARS) accumulation, increasing the GSH level and antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase. The specific DNA-binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from 50 mum and 100 mum genistein treatments were significanly suppressed. These results suggest that genistein has mild antioxidant activity to suppress intracellular oxidative stress and NFkappaB activation.