Cytokine production in whole blood cell cultures of patients with rheumatoid arthritis
Cytokine production in whole blood cell cultures of patients with rheumatoid arthritis
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DOI：
10.1136/ard.56.11.693
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发表时间：
1997-11-01
影响因子：
27.4
通讯作者：
Aarden, LA
中科院分区：
文献类型：
--
作者：
Swaak, AJG;vandenBrink, HG;Aarden, LA
目的：测定全血细胞（WBC）培养系统中活化淋巴细胞和单核细胞的细胞因子产生，为评估类风湿关节炎（RA）患者实际持续的免疫反应提供一种灵敏的工具。方法：采用高达250 pg/ml的脂多糖（LPS）刺激单核细胞，测量肿瘤坏死因子α (TNF - α)、白细胞介素6 （IL6）和il - 12的产生；采用抗cd3 （1 μ g/ml）和抗cd28 （5 μ g/ml）联合刺激T细胞，测量il - 4和干扰素γ (INF - γ)的产生。研究了27例RA患者和23例健康对照者。结果：RA患者il -6 （LPS刺激4-6 pg/ml）和il - 12 （LPS刺激16-62 pg/ml）的产生明显降低。两种细胞因子的最大产量与正常对照相当。T细胞刺激显示RA患者的干扰素γ产生显著减少。结论：在白细胞培养系统中获得的这些发现高度提示RA患者中il - 12产生减少导致TH-1衍生细胞因子产生减少。另一种可能性是白细胞中il - 12和INF - γ的产生都受到最终循环血清因子的抑制。
Objective-The measurement of cytokine production of activated lymphocytes and monocytes in the whole blood cell (WBC) culture system may provide a sensitive tool for evaluating the actual ongoing immune response of patients with rheumatoid arthritis (RA).Methods-Lipopolysaccharide (LPS) up to 250 pg/ml was used for the stimulation of monocytes for measuring the production of tumour necrosis factor alpha (TNF alpha), interleukin 6 (IL6) and IL12, while the anti-CD3 (1 mu g/ml) and anti-CD28 (5 mu g/ml) combination was used for T cell stimulation with the measuring of IL4 and interferon gamma (INF gamma) production. Twenty seven patients with RA and 23 healthy controls were studied.Results-The results showed a decreased IL6 (LPS stimulus 4-6 pg/ml) and IL12 (LPS stimulus 16-62 pg/ml) production in the RA patients. The maximal production of both cytokines was comparable with the normal controls. T cell stimulation showed a significant decreased INF gamma production in the RA patients.Conclusions-These findings obtained in the WBC culture system are highly suggestive for a decreased TH-1 derived cytokine production by a diminished IL12 production in RA patients. Another possibility is that both IL12 and INF gamma production in WBCs are inhibited by eventual circulating serum factors.