Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH
Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH
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DOI：
10.1016/s0092-8674(00)00083-0
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发表时间：
2000-09-01
期刊：
影响因子：
64.5
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Chang, WH;Kornberg, RD
来自酵母的核心TFIIH由RNA聚合酶II转录和核苷酸切除DNA修复所需的五个亚基组成，在带电脂质层上形成2D晶体。负染色晶体的电子显微照片衍射扩展到约13埃的分辨率，和3D重建显示几个离散的密度，其体积对应的个别TFIIH亚基。该结构基于三个亚基Tfb 1，Tfb 2和Tfb 3的环，其上附加了几个功能部分：Rad 3，通过SsI 1桥连到Tfb 1; SsI 2，已知与Tfb 2相互作用;和Kin 28，已知与Tfb 3相互作用。
Core TFIIH from yeast, made up of five subunits required both for RNA polymerase II transcription and nucleotide excision DNA repair, formed 2D crystals on charged lipid layers. Diffraction from electron micrographs of the crystals in negative stain extended to about 13 Angstrom resolution, and 3D reconstruction revealed several discrete densities whose volumes corresponded well with those of individual TFIIH subunits. The structure is based on a ring of three subunits, Tfb1, Tfb2, and Tfb3, to which are appended several functional moieties: Rad3, bridged to Tfb1 by SsI1; SsI2, known to interact with Tfb2; and Kin28, known to interact with Tfb3.