Villous motility and unstirred water layers in canine intestine.
Villous motility and unstirred water layers in canine intestine.
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犬肠道中的绒毛运动和未搅拌的水层。
DOI：
10.1152/ajpgi.1990.258.2.g238
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Granger,DN
中科院分区：
文献类型：
--
作者：
Mailman,D;Womack,WA;Kvietys,PR;Granger,DN
绒毛运动性通过机械搅拌减少粘膜未搅拌水层的可能性进行了检查。将一段麻醉的犬空肠或回肠保持完整的神经和血供，置于一个密封的腔室中，通过台氏液灌注，用视频显微镜技术测量绒毛的收缩频率。放射性同位素标记的菊粉，H2O，丁酸和月桂酸被用来测量净和/或单向流量从室。单向吸收运输的水和丁酸，但不月桂酸空肠段显着相关的流量通过腔室。血浆容量扩张增加绒毛运动，但减少了H2O和月桂酸的吸收。丁酸从回肠的吸收是从空肠的差异不大，虽然绒毛运动的程度较小，净吸水量更大，从回肠。丁酸吸收到死亡组织中表明，被动扩散到组织中占完整组织中吸收的7 - 25%（取决于流速），非特异性结合较低。得出的结论是，绒毛运动没有搅拌未搅拌的水层，并没有直接与改变运输。
The possibility that villous motility reduces the mucosal unstirred water layer by mechanical stirring was examined. The frequency of contraction of villi was measured by using videomicroscopic techniques while a segment of anesthetized canine jejunum or ileum with its nerve and blood supply intact was maintained in a sealed chamber through which Tyrode solution was perfused. Radioisotopically labeled inulin, H2O, and butyric and lauric acid were used to measure net and/or unidirectional fluxes from the chamber. The unidirectional absorptive transport of H2O and butyric acid but not lauric acid by jejunal segments was significantly correlated with flow through the chamber. Plasma volume expansion increased villous motility but decreased the absorption of H2O and lauric acid. Absorption of butyric acid from the ileum was little different than from the jejunum although the degree of villous motility was less and net water absorption was greater from the ileum. Absorption of butyric acid into dead tissue indicated that passive diffusion into the tissue accounted for between 7 and 25%, depending on flow rate, of the absorption in intact tissue and that nonspecific binding was low. It was concluded that villous motility did not stir the unstirred water layers and was not directly associated with altered transport.