Registration and warping of magnetic resonance images to histological sections
Registration and warping of magnetic resonance images to histological sections
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DOI：
10.1118/1.598671
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发表时间：
1999-08-01
期刊：
影响因子：
3.8
通讯作者：
Knight, RA
中科院分区：
文献类型：
--
作者：
Jacobs, MA;Windham, JP;Knight, RA
我们提出了一种磁共振图像（MRI）的配准和翘曲的组织切片比较的目的。这种方法包括一个修改后的头和帽子表面为基础的注册算法，然后由一个新的自动翘曲的方法，使用非线性薄板样条，以补偿数据集之间的失真。为了测试该方法，15只雄性Wistar大鼠通过永久性闭塞大脑中动脉进行局灶性脑缺血。在闭塞后16-24 h（n=9）和48-168 h（n=6）在不同组的动物中获得MRI图像。成像后，立即处死动物，获得苏木精和伊红染色的脑切片用于组织学分析。将MRI共配准并扭曲到组织切片。使用特征图像（EI）滤波技术定义MRI病变区域。EI是一种线性滤波器，其最大化期望组织（缺血组织）的投影，同时最小化不期望组织（非缺血组织）到称为EI的合成图像上的投影。当使用无扭曲的共配准时，MRI病变面积显示出较差的相关性（r=0.55，p>0.01），两个病变面积之间的百分比差异为22.5%+/-10.8%。扭转后MRI与组织学检查结果有显著相关性（r=0.97，p
We present a method for coregistration and warping of magnetic resonance images (MRI) to histological sections for comparison purposes. This methodology consists of a modified head and hat surface-based registration algorithm followed by a new automated warping approach using nonlinear thin plate splines to compensate for distortions between the data sets. To test the methodology, 15 male Wistar rats were subjected to focal cerebral ischemia via permanent occlusion of the middle cerebral artery. The MRI images were acquired in separate groups of animals at 16-24 h (n=9) and 48-168 h (n=6) postocclusion, After imaging, animals were immediately sacrificed and hematoxylin- and eosin-stained brain sections were obtained for histological analysis. The MRI was coregistered and warped to histological sections. The MRI lesion areas were defined using the Eigenimage (EI) filter technique. The EI is a linear filter that maximizes the projection of a desired tissue (ischemic tissue) while it minimizes the projection of undesired tissues (nonischemic tissue) onto a composite image called an EI. When using coregistration without warping the MRI lesion area demonstrated poor correlation (r=0.55, p>0.01) with a percent difference between the two lesion areas of 22.5%+/-10.8%. After warping, the MRI and histology had significant correlation (r=0.97, p