Effects of nitric oxide and tumor necrosis factor-α on production of prostaglandin F2α, and E2 in bovine endometrial cells
Effects of nitric oxide and tumor necrosis factor-α on production of prostaglandin F2α, and E2 in bovine endometrial cells
复制标题
DOI：
10.1262/jrd.50.333
复制
发表时间：
2004-06-01
影响因子：
1.8
通讯作者：
Skarzynski, DJ
中科院分区：
文献类型：
--
作者：
Woclawek-Potocka, I;Deptula, K;Skarzynski, DJ
本研究的目的是确定一氧化氮（NO）是否介导肿瘤坏死因子（TNF）α对牛子宫内膜的影响。 TNFα对牛子宫内膜的影响仅限于基质细胞。因此，了解 TNFα 刺激的基质细胞产生的 NO 是否会影响子宫内膜上皮是很有趣的。此外，我们还研究了 TNFα 和 NO 调节的前列腺素 (PG) F-2α 和 PGE(2) 合成的细胞内机制。通过酶分散法分离来自牛子宫内膜（发情周期第2-5天）的上皮细胞和基质细胞，并在48孔板中培养6-7天。将汇合的子宫内膜细胞暴露于NO供体（S-NAP；1-1000μM）24小时。 S-NAP 强烈刺激两种牛子宫内膜细胞类型中 PGE2 的产生（P
The purpose of this study was to determine whether nitric oxide (NO) mediates tumor necrosis factor (TNF)alpha influence on the bovine endometrium. TNF alpha influence on the bovine endometrium is limited to the stromal cells. Therefore, it was interesting to find out whether NO production by the stromal cells, stimulated by TNFalpha might influence the endometrial epithelium. Moreover, we investigated the intracellular mechanisms of TNFalpha- and NO-regulated prostaglandin (PG) F-2alpha and PGE(2) synthesis. Epithelial and stromal cells from the bovine endometrium (Days 2-5 of the oestrous cycle) were separated by means of enzymatic dispersion and cultured for 6-7 days in 48-well plates. The confluent endometrial cells were exposed to a NO donor (S-NAP; 1-1000 muM) for 24 h. S-NAP strongly stimulated PGE2 production in both bovine endometrial cell types (P