[Electroacupuncture of "Shangjuxu"(ST37) and "Tianshu"(ST25) reduces colonic injury by suppressing NF-kappaB/NLRP3 signaling in rats with ulcerative colitis].
[Electroacupuncture of "Shangjuxu"(ST37) and "Tianshu"(ST25) reduces colonic injury by suppressing NF-kappaB/NLRP3 signaling in rats with ulcerative colitis].
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DOI：
10.13702/j.1000-0607.20210361
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发表时间：
2022-04-25
期刊：
影响因子：
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通讯作者：
Deng, Shi-Feng
中科院分区：
文献类型：
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作者：
Cao, Qian-Ru;Ling, Chen;Deng, Shi-Feng
目的：观察电针“上巨虚”、“天枢”对结肠黏膜损伤及核因子-κ B活性的影响本研究旨在通过检测溃疡性结肠炎（UC）大鼠结肠组织中NF-κ B/Nod-like receptor family，pyrin domain-containing 3（NLRP 3）炎性小体信号通路的变化，探讨其改善UC的机制。雄性SD大鼠随机分为空白组、模型组、药物组和电针组，每组12只。采用2-4-6-三硝基苯磺酸+50%乙醇（2.5mL）灌肠建立UC模型。电针（10 Hz/50 Hz）双侧ST 37、ST 25，每次20 min，1次/d，共10 d。给药组大鼠灌胃美沙拉秦混悬液（2 mL：0.2g/kg+0.9%生理盐水），每日1次，连续10 d。记录大鼠一般情况，计算疾病活动指数（DAI）评分（0-4分）。取结肠组织行结肠黏膜损伤指数（CMDI，0-5分）评分，HE染色观察组织病理学变化，免疫组化和Western blot检测NF-κ B和NLRP 3的表达水平。结果：与空白组比较，模型组大鼠DAI、CMDI评分、血清IL-1 β、NLRP 3、TNF-α含量、免疫活性及NF-κ B、NLRP 3蛋白表达均显著升高（P
OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Shangjuxu"(ST37) and "Tianshu"(ST25) on colonic mucosal injury and activities of nuclear factor-kappaB (NF-kappaB)/Nod-like receptor family，pyrin domain-containing 3 (NLRP3) inflammasome signaling in the colonic tissue in rats with ulcerative colitis (UC), so as to explore its mechanisms underlying improvement of UC.METHODS: Male SD rats were randomly divided into blank, model, medication and EA groups, with 12 rats in each group. The UC model was established by enema of 2-4-6 trinitrobenzene sulfonic acid +50% ethanol (2.5 mL). EA (10 Hz/50 Hz) was applied to bilateral ST37 and ST25 for 20 min, once a day, for a total of 10 days. Rats of the medication group received gavage of mesalazine suspension (2 mL：0.2 g/kg+0.9% saline) once a day, for 10 days. The rats' general conditions were recorded for calculating the disease activity index (DAI) score (0-4 points). The colonic tissue was sampled for giving colonic mucosa damage index (CMDI, 0-5 points) score and for observing histopathological changes after hematoxylin-eosin (HE) staining, and for detecting expression levels of NF-kappaB and NLRP3 by using immunohistochemistry and Western blot, separately. The contents of serum interleukin-1beta (IL-1beta), NLRP3 and tumor necrosis factor-alpha (TNF-alpha) were detected by enzyme-linked immunosorbent assay.RESULTS: Compared with the blank group, the DAI and CMDI scores, contents of serum IL-1beta, NLRP3, and TNF-alpha, as well as the immunoactivity and expression of NF-kappaB and NLRP3 proteins were significantly increased in the model group (P