Apoptotic death of olfactory sensory neurons in the adult rat
Apoptotic death of olfactory sensory neurons in the adult rat
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DOI：
10.1006/exnr.1996.6352
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发表时间：
1997-01-01
影响因子：
5.3
通讯作者：
Frisen, J
中科院分区：
文献类型：
--
作者：
Deckner, ML;Risling, M;Frisen, J
在大多数哺乳动物中，嗅觉神经元只能存活一个月左右。目前还不完全清楚这些神经元的短寿命是否是由于坏死性死亡，或者这些细胞是否通过凋亡而死亡。经历凋亡性细胞死亡的细胞的一个特征是核小体间DNA片段化。我们已经使用TdT介导的dUTP-地高辛缺口末端标记（TUNEL）检测细胞进行DNA片段化原位。在完整的成年大鼠嗅上皮的基底未成熟的神经元祖细胞的亚群，以及成熟的嗅觉感觉神经元，表现出DNA片段。TUNEL标记的神经元的数量显着增加后1.5天切断的嗅丝和下降到对照组的水平后4天的损伤。为了将DNA片段化与细胞凋亡的超微结构特征联系起来，我们修改了TUNEL标记协议，以便在电子显微镜下研究TUNEL标记的细胞。这证实了TUNEL标记的神经元具有凋亡的形态学特征。这些数据为成年哺乳动物神经系统中神经元的凋亡性死亡提供了证据。嗅觉感觉神经元的营业额，至少部分地，由细胞凋亡和破坏的接触与嗅球的结果在嗅上皮中的神经元的大量凋亡性死亡的调节。(C)1997学术出版社
Olfactory sensory neurons only live for about I month in most mammals. It is not fully understood whether the short life span of these neurons is due to necrotic death, or if these cells die by apoptosis. One characteristic of cells undergoing apoptotic cell death is internucleosomal DNA-fragmentation. We have used TdT-mediated dUTP-digoxigenin nick end labeling (TUNEL) to detect cells undergoing DNA-fragmentation in situ. In the intact olfactory epithelium of adult rats a subpopulation of basal immature neuronal progenitor cells, as well as mature olfactory sensory neurons, showed DNA-fragmentation. The number of TUNEL-labeled neurons increased dramatically 1.5 days after transection of the fila olfactoria and declined to control levels by Day 4 after the injury. In order to relate DNA-fragmentation to ultrastructural characteristics of apoptosis we modified the TUNEL-labeling protocol to enable studies of TUNEL-labeled cells in the electron microscope. This confirmed that TUNEL-labeled neurons showed morphological characteristics of apoptosis. The data provide evidence for apoptotic death of neurons in the adult mammalian nervous system. The turnover of olfactory sensory neurons is, at least in part, regulated by apoptosis and disruption of the contact with the olfactory bulb results in massive apoptotic death of neurons in the olfactory epithelium. (C) 1997 Academic Press