HERBASPIRILLUM-LIKE BACTERIA IN BANANA PLANTS
HERBASPIRILLUM-LIKE BACTERIA IN BANANA PLANTS
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香蕉植物中的草本螺菌样细菌
DOI：
10.1590/s1517-83822001000300007
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
J. Döbereiner
中科院分区：
文献类型：
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作者：
O. B. Weber;L. M. Cruz;J. Baldani;J. Döbereiner
从香蕉植株上分离到一株固氮细菌，对其进行了形态学和生理学研究。这些植物细菌可以建立三个不同的群体。其中两个与Herbaspirillum属的物种相似。第三个是不同的，因为只使用了几个碳基板，并产生水扩散的化合物，在紫外光下发出荧光。3个菌群均为单鞭毛或双鞭毛细杆状，革兰氏染色阴性，具有过氧化氢酶活性，能还原硝酸盐，在31 ℃的半固体JNFb培养基中生长较好。在半固体无氮JNFb培养基中检测到固氮酶活性，当pH范围为6.5至7.0（组I和II）和6.0至6.5（组III）时表达较高的值。从香蕉植株中分离的固氮菌与先前在禾本科植物中鉴定的Herbaspirillum不同。
Diazotrophic bacteria isolated from banana plants were characterized by morphological and physiological aspects. Three different groups of these plant-bacteria could be established. Two of them showed similarity to species of the Herbaspirillum genus. The third one was different because used only a few carbon substrates and produced water diffusible compounds that fluoresced under UV light. All three bacterial groups were thin rods with mono or bipolar flagella, presented negative reaction in Gram stain, showed catalase activity, were able to reduce nitrate and grew better in semi-solid JNFb medium at 31oC. The nitrogenase activity was detected in semi-solid N-free JNFb medium and expressed higher values when pH ranged from 6.5 to 7.0 (groups I and II) and 6.0 to 6.5 (group III). The diazotrophs isolated from banana plants were distinct from species of Herbaspirillum previously identified in gramineous plants.