Enantioselective effects of imazethapyr residues on Arabidopsis thaliana metabolic profile and phyllosphere microbial communities
Enantioselective effects of imazethapyr residues on Arabidopsis thaliana metabolic profile and phyllosphere microbial communities
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咪草烟残留物对拟南芥代谢谱和叶际微生物群落的对映选择性影响
DOI：
10.1016/j.jes.2020.03.009
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发表时间：
2020
影响因子：
6.9
通讯作者：
Qian Haifeng
中科院分区：
文献类型：
--
作者：
Zhao Qianqiu;Liu Wanyue;Li Yan;Ke Mingjing;Qu Qian;Yuan Wenting;Pan Xiangliang;Qian Haifeng
咪草烟 (IM) 是一种广泛使用的乙酰乳酸合酶抑制手性除草剂。它具有长期残留，可能通过植物的可食用部分（例如蔬菜叶子或水果）被人体吸收。在这里，我们选择了一种模式植物拟南芥，以确定R-IM和S-IM在暴露7天后对其叶片结构、光合效率和代谢产物以及叶际微生物结构的影响。我们的结果表明 R-IM 和 S-IM 之间植物生长的对映体差异； 133 µg/kgR-IM比S-IM对光合效率的抑制更重，对亚细胞结构的改变也更大。R-IM和S-IM对代谢和叶片微生物也有不同的影响。S-IM主要增加脂质化合物和减少氨基酸，而R-IM增加糖积累。 R-IM处理增加了莫拉氏菌科人类致病菌的相对丰度，表明R-IM处理可能增加了叶面致病菌的含量。我们的研究为评估土壤中农药残留的危害性、通过植物代谢调节引起的叶际微生物组变化以及诱发病原菌积累的风险提供了新的视角。
Imazethapyr (IM) is a widely used acetolactate synthase-inhibiting chiral herbicide. It has long-term residuals that may be absorbed by the human body through the edible parts of plants, such as vegetable leaves or fruits. Here, we selected a model plant,Arabidopsis thaliana, to determine the effects ofR-IM andS-IM on its leaf structure, photosynthetic efficiency, and metabolites, as well as the structures of microorganisms in the phyllosphere, after 7 days of exposure. Our results indicated enantiomeric differences in plant growth betweenR-IM andS-IM; 133 µg/kgR-IM showed heavier inhibition of photosynthetic efficiency and greater changes to subcellular structure thanS-IM.R-IM andS-IM also had different effects on metabolism and leaf microorganisms.S-IM mainly increased lipid compounds and decreased amino acids, whileR-IM increased sugar accumulation. The relative abundance ofMoraxellaceaehuman pathogenic bacteria was increased byR-IM treatment, indicating thatR-IM treatment may increase leaf surface pathogenic bacteria. Our research provides a new perspective for evaluating the harmfulness of pesticide residues in soil, phyllosphere microbiome changes via the regulation of plant metabolism, and induced pathogenic bacterial accumulation risks.