Identification of a human hepatocellular carcinoma-associated tumor suppressor gene by differential display polymerase chain reaction.
Identification of a human hepatocellular carcinoma-associated tumor suppressor gene by differential display polymerase chain reaction.
复制标题
通过差异显示聚合酶链反应鉴定人肝细胞癌相关抑癌基因。
DOI：
10.1016/0024-3205(95)02053-l
复制
发表时间：
1995
期刊：
影响因子：
6.1
通讯作者：
Carr,BI
中科院分区：
文献类型：
--
作者：
Wu,GS;Kar,S;Carr,BI
应用差异显示聚合酶链式反应技术研究了人正常肝脏和人肝细胞癌细胞系之间的差异基因表达。克隆并测序了一个在肝癌细胞系中与正常肝脏相比表达下调的基因(线粒体蛋白脂样基因，MPL)。从核苷酸序列翻译而来的氨基酸序列与从牛心分离的线粒体蛋白脂(MPLP)的羧基末端有73%的同源性。Northern印迹分析显示，在45例人肝细胞癌中有20例(44%)未检测到3kb MPL转录本的表达，而在14例非肝细胞癌中只有1例(17%)与正常肝组织相比未检测到mpl转录本的表达。因此，MPL可能是人肝细胞癌的候选抑癌基因。MPL表达的下降并不是由于其基因组DNA的大体改变。
Differential gene expression between the normal human liver and a cell line derived from human hepatocellular carcinoma (HCC) was studied using the differential display polymerase chain reaction technique. One gene (mitochondria proteolipid like gene, MPL), whose expression was found to be repressed in the HCC cell line compared to normal liver, was cloned and sequenced. Amino acid sequence translated from the nucleotide sequence had a 73% homology with the carboxyl terminus of a mitochondria proteolipid (MPLP) isolated from beef heart. Northern blot analysis showed that the expression of the 3 kb MPL transcript was undetectable in 20 of 45 (44%) of human hepatocellular carcinomas, whereas only 1 of 14 (17%) of cirrhotic livers without HCC had undetectable expression when compared to normal livers. Hence MPL may be a candidate tumor suppressor gene for human HCC. This decrease in MPL expression was not due to gross alteration of its genomic DNA.