Inhibitory effect of puerarin on vascular smooth muscle cells proliferation induced by oxidised low-density lipoprotein via suppressing ERK 1/2 phosphorylation and PCNA expression
Inhibitory effect of puerarin on vascular smooth muscle cells proliferation induced by oxidised low-density lipoprotein via suppressing ERK 1/2 phosphorylation and PCNA expression
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DOI：
10.1691/ph.2016.5703
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发表时间：
2016-02-01
期刊：
影响因子：
1.6
通讯作者：
Ren, Liqun
中科院分区：
文献类型：
--
作者：
Hu, Yanwu;Liu, Kai;Ren, Liqun
葛根素（Puerarin）是从传统中草药葛根（Pueraria lobata（Wild.）Ohwi已被证明在体内和体外具有抗氧化、抗炎、抗癌、抗高胆固醇血症和抗高血糖活性。本研究旨在探讨葛根素对氧化低密度脂蛋白（ox-LDL）刺激的血管平滑肌细胞（VSMCs）增殖的抑制作用及其可能机制。用不同浓度的葛根素（0、1、10、50 μ M）预处理血管平滑肌细胞，然后用ox-LDL（50 μ g/mL）刺激。MTT法检测细胞增殖;流式细胞仪检测葛根素对细胞周期的影响。Western blotting检测细胞增殖细胞核抗原（PCNA）表达和细胞外信号调节激酶（ERK）1/2磷酸化水平。结果表明，葛根素能显著抑制ox-LDL诱导的VSMCs增殖和ERK 1/2磷酸化。葛根素还可阻断ox-LDL诱导的VSMCs细胞周期G1/S间期进程，下调PCNA表达。结果提示葛根素通过抑制ERK 1/2磷酸化和PCNA表达抑制ox-LDL诱导的VSMCs增殖。
Puerarin, an isoflavonoid isolated from the traditional Chinese herbal medicine Pueraria lobata (Wild.) Ohwi, has been shown to process antioxidant, anti-inflammatory, anti-cancer, anti-hypercholesterolemic, and anti-hyperglycemic activities in vivo and in vitro. The aim of the present study was to investigate the antiproliferative effects and the possible mechanisms of puerarin in vascular smooth muscle cells (VSMCs) stimulated with oxidised low-density lipoprotein (ox-LDL). VSMCs were cultured and pretreated with different concentrations of puerarin (0, 1, 10, 50 mu M) before stimulated by ox-LDL (50 mu g/mL). Cell proliferation was evaluated by MTT assay. Flow cytometry was used to study the influence of puerarin on cell cycle. Proliferating cell nuclear antigen (PCNA) expression and phosphorylation levels of extracellular signal-regulated kinase (ERK) 1/2 were detected by western blotting analysis. The results indicated that puerarin significantly inhibited VSMCs proliferation induced by ox-LDL and phosphorylation of ERK 1/2. Furthermore, puerarin also blocked the ox-LDL-induced cell-cycle progression at G1/S-interphase and down-regulated the expression of PCNA of VSMCs. The results suggest puerarin inhibits ox-LDL-induced proliferation of VSMCs by suppressing ERK 1/2 phosphorylation and PCNA expression.