Mouse vaginal opening is an apoptosis-dependent process which can be prevented by the overexpression of Bcl2
Mouse vaginal opening is an apoptosis-dependent process which can be prevented by the overexpression of Bcl2
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DOI：
10.1006/dbio.1997.8522
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发表时间：
1997-04-01
影响因子：
2.7
通讯作者：
Vassalli, P
中科院分区：
文献类型：
--
作者：
Rodriguez, I;Araki, K;Vassalli, P
在老鼠身上，阴道向皮肤开放是一个较晚的事件，大约发生在生命的第五周左右；它可以通过注射-雌二醇在性成熟小鼠中诱导。我们已经培育了两种在多种组织中表达人Bcl2蛋白的转基因小鼠。转基因雌性小鼠的阴道永远关闭，完全抵抗β -雌二醇注射；这伴有生殖道的相当大的膨胀。正常小鼠阴道切开时的组织学研究表明，通过TUNEL方法可以检测到DNA片段的细胞核。在bcl2转基因小鼠中，在阴道粘膜基底上上皮细胞和上皮下细胞中表达bcl2的小鼠阻止了这一过程。相比之下，在K10角蛋白启动子的控制下，携带Bcl2转基因的两种小鼠具有正常的表型，K10角蛋白启动子的表达仅限于表皮的基底上层。在这些转基因品系中，眼睑的形成和外耳道的打开（在小鼠出生后也会发生）都没有改变；在这些事件发生时的眼和耳切片的组织学研究未能检测到细胞凋亡。总之，小鼠雌性生殖道在青春期完成成熟所需的组织重塑是一个激素触发的细胞凋亡依赖过程。(C) 1997学术出版社。
In the mouse, opening of the vaginal cavity to the skin is a late event, occurring around the fifth week of life; it can be induced in sexually immature mice by beta-estradiol injections. We have generated two lines of transgenic mice expressing the human Bcl2 protein in a variety of tissues. The vaginal cavity of the transgenic females remained permanently closed, a condition completely resistant to beta-estradiol injections; this was accompanied by a considerable distension of the genital tract. Histologic studies of vaginal sections at the time of opening to the skin in normal mice showed, by the TUNEL method which detects nuclei with fragmented DNA. characteristic of apoptosis, that this event coincides with extensive apoptosis in the lower part of the vaginal mucosa, a process prevented in the bcl2 transgenic mice, which express Bcl2 in suprabasal epithelial cells and in subepithelial cells of the vaginal mucosa. In contrast, two lines of mice bearing a Bcl2 transgene placed under the control of a K10 keratin promoter, whose expression is restricted to the suprabasal layers of the epidermis, had a normal phenotype. Eyelids' formation and opening of the external ear canals, which also occur after birth in the mouse, were not altered in any of these transgenic lines; histological study of eye and ear sections at the time of these events failed to detect apoptosis. In conclusion, the tissue remodeling required to complete maturation of the mouse female genital tract at the time of puberty is an hormonally triggered apoptosis-dependent process. (C) 1997 Academic Press.