Mutanolysin, Bacteriolytic Agent for Cariogenic Streptococci: Partial Purification and Properties
Mutanolysin, Bacteriolytic Agent for Cariogenic Streptococci: Partial Purification and Properties
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DOI：
10.1128/aac.6.2.156
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发表时间：
1974-08
影响因子：
4.9
通讯作者：
K. Yokogawa;S. Kawata;S. Nishimura;Y. Ikeda;Y. Yoshimura
中科院分区：
文献类型：
--
作者：
K. Yokogawa;S. Kawata;S. Nishimura;Y. Ikeda;Y. Yoshimura
从globisporus Streptomyces globisporus 1829培养滤液中部分纯化得到的变酵素由两种主要的裂解酶组成，其等电点分别在pH 8.5和10附近，其中蛋白水解酶与后一种裂解酶相关联。在pH值为6.5 ~ 7.0的60℃条件下，突变解菌素的裂解活性最大，在酸度为50℃的条件下，突变解菌素的裂解活性稳定。n -溴代琥珀酰亚胺在1 mM时对酶解活性有完全抑制作用，而相同浓度的钙、镁离子对酶解活性有激活作用。对变形链球菌、唾液链球菌、血链球菌、嗜酸乳杆菌和粘胶放线菌的活细胞均有溶解或杀菌作用，但对金黄色葡萄球菌和所有革兰氏阴性菌株均无活性。酶解活性在人唾液中保持良好。突变解素对变形链球菌BHT细胞壁的消化伴随着游离氨基和还原糖的释放。溶变素有望成为一种防治龋齿的有效药物。
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal lytic activity at 60 C in the pH range 6.5 to 7.0 and was stable at 50 C in the acid range. N-bromosuccinimide caused complete inhibition of lytic activity at 1 mM, whereas calcium and magnesium ions at the same concentration caused activation. Mutanolysin had lytic or bactericidal activity against the living cells of Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, Lactobacillus acidophilus, and Actinomyces viscosus, which are considered to be etiologic agents of dental caries, but had no activity against S. aureus and all gram-negative strains tested. The lytic activity was well retained in human saliva. Digestion of the cell walls of S. mutans BHT by mutanolysin was accompanied by the liberation of free amino groups and reducing sugars. Mutanolysin may be expected to be a useful agent for dental caries control.