Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro
Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro
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DOI：
10.1111/rda.12730
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发表时间：
2016-10-01
影响因子：
1.7
通讯作者：
Schneider, A.
中科院分区：
文献类型：
--
作者：
Rincon, J. A. A.;Madeira, E. M.;Schneider, A.
对氧磷酶-1（PON 1）是一种在血清和卵泡液中发现的酶，可保护细胞膜和循环脂质免受氧化损伤。本研究的目的是在分子水平（基因表达）上测量重组PON 1（rPON 1）对牛卵母细胞成熟的直接影响，并测量PON 1对体外植入前胚胎发育的遗留效应。将COC提交至IVM，加入0.0、0.02、0.04和0.08 mg/ml rPON 1，分别对应于2.2 +/- 0.4、15.5 +/- 1.5、30.2 +/- 3.0和57.9 +/- 5.0 U/ml的平均PON 1芳基酯酶活性。结果表明，在IVM期间添加rPON 1以剂量依赖性方式改善胚胎发育，因为处理组的D 7胚胎发育分别为22.2%、29.4%、32.2%和37.0%（p = 0.02）。总之，在IVM过程中添加PON 1酶对胚胎发育至囊胚的速率产生了剂量相关的积极影响。
Paraoxonase-1 (PON1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON1 (rPON1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry-over effects of PON1 on pre-implantation embryo development in vitro. COCs were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml(-1) of rPON1, corresponding to an average PON1 arylesterase enzyme activity of 2.2 +/- 0.4, 15.5 +/- 1.5, 30.2 +/- 3.0 and 57.9 +/- 5.0 U ml(-1), respectively. The results indicated that addition of rPON1 during IVM improved embryo development in a dose-dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively (p = 0.02). In conclusion, addition of PON1 enzyme during IVM exerted dose-related positive effects on embryo development rates to blastocysts.