Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip
Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip
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DOI：
10.1016/j.ultrasmedbio.2006.07.024
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Wiklund, M.
中科院分区：
文献类型：
--
作者：
Hultstrom, J.;Manneberg, O.;Wiklund, M.
超声驻波(USW)技术有可能成为微流控芯片中温和和非接触细胞处理的标准方法。我们通过研究超声捕获和微流控芯片中低数量细胞聚集后再培养细胞的增殖率来研究贴壁细胞暴露在UWS中的活性。细胞在芯片内形成二维聚集体，聚集体被保持在垂直于驻波传播方向的细胞培养介质的连续流动中。在声压为0.85兆帕、时间为30~75min的情况下，保存在陷阱中的COS-7细胞的倍增时间与预期值(24~48h)没有偏差。因此，这些结果证明了超声驻波作为一种工具在微流控系统中温和地处理低细胞数的潜力。(电子邮件：jessica.hultstrom@biox.kth.se)(C)2006年世界医学和生物学超声联合会。
Ultrasonic-standing-wave (USW) technology has potential to become a standard method for gentle and contactless cell handling in microfluidic chips. We investigate the viability of adherent cells exposed to USWs by studying the proliferation rate of recultured cells following ultrasonic trapping and aggregation of low cell numbers in a microfluidic chip. The cells form 2-D aggregates inside the chip and the aggregates are held against a continuous flow of cell culture medium perpendicular to the propagation direction of the standing wave. No deviations in the doubling time from expected values (24 to 48 h) were observed for COS-7 cells held in the trap at acoustic pressure amplitudes up to 0.85 MPa and for times ranging between 30 and 75 min. Thus, the results demonstrate the potential of ultrasonic standing waves as a tool for gentle manipulation of low cell numbers in microfluidic systems. (E-mail: jessica.hultstrom@biox.kth.se) (c) 2006 World Federation for Ultrasound in Medicine & Biology.