Preclinical model to test human papillomavirus virus (HPV) capsid vaccines in vivo using infectious HPV/cottontail rabbit papillomavirus chimeric papillomavirus particles
Preclinical model to test human papillomavirus virus (HPV) capsid vaccines in vivo using infectious HPV/cottontail rabbit papillomavirus chimeric papillomavirus particles
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DOI：
10.1128/jvi.01583-06
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发表时间：
2006-12-01
影响因子：
5.4
通讯作者：
Christensen, Neil D.
中科院分区：
文献类型：
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作者：
Mejia, Andres F.;Culp, Timothy D.;Christensen, Neil D.
一种由病毒样颗粒（vlp）组成的人乳头瘤病毒（HPV）疫苗最近被批准用于人。一般认为，VLP疫苗通过诱导类型特异性中和抗体来保护。由于物种限制，临床前动物模型不能用于检测对HPV感染的保护。我们建立了一种使用嵌合HPV衣壳/棉尾兔乳头瘤病毒（CRPV）基因组颗粒的模型，以允许在兔中直接测试HPV VLP疫苗。接种了CRPV、HPV 16型（HPV-16）或HPV-11 VLPs的动物被同源（CRPV衣壳）和嵌合（HPV-16衣壳）颗粒攻击。观察到强类型特异性保护，证明了该方法的潜在应用。
A human papillomavirus (HPV) vaccine consisting of virus-like particles (VLPs) was recently approved for human use. It is generally assumed that VLP vaccines protect by inducing type-specific neutralizing antibodies. Preclinical animal models cannot be used to test for protection against HPV infections due to species restriction. We developed a model using chimeric HPV capsid/cottontail rabbit papillomavirus (CRPV) genome particles to permit the direct testing of HPV VLP vaccines in rabbits. Animals vaccinated with CRPV, HPV type 16 (HPV-16), or HPV-11 VLPs were challenged with both homologous (CRPV capsid) and chimeric (HPV-16 capsid) particles. Strong type-specific protection was observed, demonstrating the potential application of this approach.