Autofluorescence Imaging of Living Yeast Cells with Deep-Ultraviolet Surface Plasmon Resonance
Autofluorescence Imaging of Living Yeast Cells with Deep-Ultraviolet Surface Plasmon Resonance
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DOI：
10.3390/photonics9060424
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发表时间：
2022-06
期刊：
影响因子：
2.4
通讯作者：
C. N. H. Che Lah;H. Morisawa;K. Kobayashi;A. Ono;W. Inami;Y. Kawata
中科院分区：
文献类型：
--
作者：
C. N. H. Che Lah;H. Morisawa;K. Kobayashi;A. Ono;W. Inami;Y. Kawata
利用深紫外表面等离子体共振（deep-UVSPR）技术在铝薄膜上激发活细胞的自发荧光。利用蓝宝石棱镜，以Kretschmann组态激发了水介质中的深紫外表面等离子体共振。深紫外表面等离子体共振是一种无需染色的高灵敏度活细胞自体荧光成像技术。无标记观察对于活细胞的结构分析具有重要意义。我们展示了高灵敏度的自发荧光成像的活酵母细胞与深紫外SPR。我们采用一种适合于水介质中样品观察的高折射率棱镜，如蓝宝石，激发深紫外SPR。虽然活细胞的典型自发荧光被背景噪声所掩盖，但深紫外SPR增强了自发荧光信号。从理论和实验上研究了铝薄膜通过蓝宝石棱镜的深紫外表面等离子体共振激发。结果表明，荧光强度增加了2.8倍。深紫外SPR增强了细胞结构的自发荧光，酵母细胞被发现是非常敏感的。因此，对于水浸样品，基于棱镜的Kretschmann配置在深紫外激发SPR。这项研究的结果表明，深紫外SPR可以被认为是一种有效的技术，实现高灵敏度的生物样品的观察。
Autofluorescence in living cells on aluminum thin film was excited with deep-ultraviolet surface plasmon resonance (deep-UV SPR). Deep-UV SPR under aqueous medium was excited with Kretschmann configuration by using a sapphire prism. Deep-UV SPR is one of the promising techniques for high-sensitive autofluorescence imaging of living cells without staining. Label-free observation is significant for the structural analysis of living cells. We demonstrated the high-sensitive autofluorescence imaging of living yeast cells with deep-UV SPR. We applied a high refractive index prism, such as sapphire, which is suitable for the observation of specimens in aqueous medium, to excite deep-UV SPR. Although typical autofluorescence from living cells is buried in background noise, deep-UV SPR enhances the autofluorescence signal. The deep-UV SPR excitation of an aluminum thin film through a sapphire prism was investigated theoretically and experimentally. It showed that the fluorescence intensities are increased 2.8-fold. Deep-UV SPR enhanced the autofluorescence of cell structures, and yeast cells were found to be very sensitive. As a result, for water-immersed specimens, the sapphire-prism-based Kretschmann configuration excited SPR in deep-UV. Findings from this study suggest that deep-UV SPR can be considered an effective technique for attaining high-sensitivity observation of biological samples.