Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungus Pleurotus ostreatus
Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungus Pleurotus ostreatus
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DOI：
10.1007/bf00833416
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Kuwahara, M
中科院分区：
文献类型：
--
作者：
Irie, T;Honda, Y;Kuwahara, M
我们从白腐菌Pleumostreatus中分离到一个编码琥珀酸脱氢酶铁硫蛋白（Ip）亚基的结构基因sdi 1。1.3.99.1在这里，我们报告分离的启动子区域的sdi 1和等位基因序列编码的第二种类型的cDNA片段在以前的实验中分离。启动子区的核苷酸序列分析揭示了假定的CAAT和TATA盒的存在，这使我们能够在这个物种中开发一个表达系统。Southern杂交分析和限制性片段长度多态性分析表明，在糙皮侧耳的单倍体基因组中不存在sdi 1家族基因。此外，还建立了一个遗传分析，以检测突变菌株中sdi 1基因型与氟酰胺抗性之间的连锁。
We have isolated a structural gene of sdi1, which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1), from a white-rot basidiomycete, Pleurotus ostreatus. Here we report isolation of the promoter region of sdi1 and an allelic sequence encoding the second-type cDNA fragment isolated in the former experiments. The nucleotide sequence analysis of the promoter region revealed the existence of putative CAAT and TATA boxes, which permits us to develop an expression system in this species. The Southern blot analysis and the restriction fragment length polymorphism assay using monokaryotic strains demonstrated that no family genes to sdi1 exist in the haploid genome of P. ostreatus. Moreover, a genetic analysis to detect a linkage between the sdi1 genotypes and flutolanil resistance in the mutant P. ostreatus strains was also developed.