Cardiomyocytes derived from pluripotent stem cells: progress and prospects from China.
Cardiomyocytes derived from pluripotent stem cells: progress and prospects from China.
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DOI：
10.1016/j.yexcr.2012.09.011
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发表时间：
2013-01
影响因子：
3.7
通讯作者：
Huang-Tian Yang;Min Zhang;Jijun Huang;He Liang;Peng Zhang;K. Boheler
中科院分区：
文献类型：
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作者：
Huang-Tian Yang;Min Zhang;Jijun Huang;He Liang;Peng Zhang;K. Boheler
胚胎干细胞或诱导多能干细胞衍生的心肌细胞（CMs）移植是治疗心肌梗死和心力衰竭的一种有前途的方法。来自这些多能干细胞（PSCs）的心脏分化系统也可以用于更好地理解早期发育生物学、药物发现、毒理学测试和疾病建模。实现这些目标的先决条件是以有效和可靠的方式生成功能性CMs的能力。还必须克服CM成熟不足的问题，并且必须优化将PSC-CMs引入心脏同时保持细胞活力的适当方法。在过去的几年中，在这些细胞的分化、表征和应用于生物系统方面取得了重大进展。在这里，我们回顾了最近的进展，特别是在中国进行的，包括通过PSC分化的心脏发生和CMs的基础干细胞生物学研究，室特异性CM分化的方法，涉及细胞内Ca2+信号调节的成熟过程，以及应用。
Transplantation of embryonic stem cell- or induced pluripotent stem cell-derived cardiomyocytes (CMs) represents one promising approach for the treatment of myocardial infarction and failing hearts. Cardiac differentiation systems from these pluripotent stem cells (PSCs) can also be employed to better understand early developmental biology, drug discovery, toxicology testing, and disease modeling. A prerequisite to attain these goals is the ability to generate functional CMs in an efficient and reliable way. The lack of CM maturation must also be overcome, and appropriate methods for introducing PSC-CMs into heart while maintaining cell viability must be optimized. The past few years have seen major advances both in the differentiation, characterization and application of these cells to biological systems. Here we review recent progress, especially those performed in China, in basic stem cell biology involving studies of cardiogenesis and CMs through PSC differentiation, approaches for chamber-specific CM differentiation, maturation processes involving regulation of intracellular Ca2+signals, and applications.