A toolkit for high-throughput, cross-species gene engineering in Drosophila
A toolkit for high-throughput, cross-species gene engineering in Drosophila
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DOI：
10.1038/nmeth.1334
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发表时间：
2009-06-01
期刊：
影响因子：
48
通讯作者：
Tomancak, Pavel
中科院分区：
文献类型：
--
作者：
Ejsmont, Radoslaw K.;Sarov, Mihail;Tomancak, Pavel
我们为黑腹果蝇和拟暗果蝇产生了两个互补的基因组fosmid文库，其允许通过高通量重组工程和直接转基因对大基因组克隆进行无缝修饰。Fosmid转基因概括了内源基因表达模式。这些文库与重组工程技术相结合，将有助于拯救突变表型，允许活蝇中的基因产物成像，并能够系统地分析和操纵跨物种的基因活性。
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.