Investigating SARS-CoV-2 persistent contamination in different indoor environments.
Investigating SARS-CoV-2 persistent contamination in different indoor environments.
复制标题
DOI：
10.1016/j.envres.2021.111763
复制
发表时间：
2021-11
影响因子：
8.3
通讯作者：
Song J
中科院分区：
文献类型：
--
作者：
Liu H;Fei C;Chen Y;Luo S;Yang T;Yang L;Liu J;Ji X;Wu W;Song J
COVID-19患者造成的环境污染可能是传播媒介。以前关于SARS-CoV-2在环境表面的报告是关于短期污染的。本研究调查了SARS-CoV-2 RNA在室温和低温环境中暴露后长时间（>28天）的存在情况。2020年1月发生COVID-19疫情的百货公司（43名COVID-19患者的震中）和患者公寓分别被封锁57天和48天后被纳入室温环境。七个冷库和里面的进口冷冻食品被列为低温环境（-18 °C以下）。此外，亦包括20个可能受进口冷冻食品污染的食品市场，以研究持续污染的情况。收集并分析了有关温度、相对湿度和自上次暴露以来环境样本的天数的信息。综上所述，消毒前共采集拭子样本11,808份，其中35份阳性。在公寓（6/19）、百货商店（3/50）、冷藏库中的食品包装（23/1360）、冷藏库的环境表面（2/345）和食品市场中的包装（1/10，034）中鉴定出SARS-CoV-2 RNA的持续污染。从公寓卫生间分离出2份阳性样品（66.7%，2/3），公寓门把手和冷库门把手分别有40%（2/5）和33.3%（1/3）的污染。对1例进口冷冻食品相关病例（天津市第138例COVID-19患者）的流行病学信息和环境污染结果进行分析。根据Ct值，通过标准曲线和线性回归计算两个靶基因的拷贝数。总之，SARS-CoV-2 RNA可以在最后一次暴露后至少57天在室温环境中检测到，比以前的报告长得多。根据本研究结果和以往的研究，传染性SARS-CoV-2可以在冷链食品包装表面存在至少60天。门把手和厕所（浴室）是COVID-19控制的重要位置。冷链相关物流作业的高危人群，如搬运工等，需要严格的防范和高水平的个人防护。
Environmental contamination caused by COVID-19 patients could be a medium of transmission. Previous reports of SARS-CoV-2 in environmental surfaces were about short-term contamination. This study investigated SARS-CoV-2 RNA existence in room-temperature and low-temperature environments long after exposure (>28 days). A department store, where a COVID-19 outbreak was occurred in January 2020 (the epicenter of 43 COVID-19 patients), and a patient's apartment were included as room-temperature environments after being blocked for 57 days and 48 days, respectively. Seven cold storages and imported frozen foods inside were included as low-temperature environments (under −18 °C). Twenty food markets with potential contamination of imported frozen foods were also included to study the consecutive contamination. Information about temperature, relative humidity, and the number of days of environmental samples since the last exposure was collected and analyzed. In sum, 11,808 swab samples were collected before disinfection, of which 35 samples were positive. Persistent contamination of SARS-CoV-2 RNA was identified in the apartment (6/19), the department store (3/50), food packages in cold storages (23/1360), environmental surfaces of cold storages (2/345), and a package in the food market (1/10,034). Two positive samples were isolated from the bathroom of the apartment (66.7 %, 2/3), and doorknobs were proved with contamination in the apartment (40 %, 2/5) and cold storage (33.3 %, 1/3). The epidemiology information and environmental contamination results of an imported frozen food related COVID-19 case (138th COVID-19 patient in Tianjin) were analyzed. Based on the Ct values, the number of copies of two target genes was calculated by standard curves and linear regressions. In conclusion, SARS-CoV-2 RNA can be detected in room-temperature environments at least 57 days after the last exposure, much longer than previous reports. Based on the results of this study and previous studies, infectious SARS-CoV-2 could exist for at least 60 days on the surface of cold-chain food packages. Doorknobs and toilets (bathrooms) were important positions in COVID-19 control. High-risk populations of cold-chain-related logistic operations, such as porters, require strict prevention and high-level personal protection.
登录
查看更多内容
影响因子：
9.8
作者：
Hu, Xiaowen;Xing, Yuhan;Jiang, Fachun
通讯作者：
Jiang, Fachun
控制因进口冷冻食品和食品包装受污染而导致的 COVID-19 传播。
影响因子：
4.7
作者：
Bai L;Wang Y;Wang Y;Wu Y;Li N;Liu Z
通讯作者：
Liu Z
影响因子：
4.7
作者：
Azuma K;Yanagi U;Kagi N;Kim H;Ogata M;Hayashi M
通讯作者：
Hayashi M
影响因子：
8.9
作者：
Carraturo, Federica;Del Giudice, Carmela;Guida, Marco
通讯作者：
Guida, Marco
影响因子：
9.8
作者：
Auler, A. C.;Cassaro, F. A. M.;Pires, L. F.
通讯作者：
Pires, L. F.