Neuropeptide Y modulates membrane excitability in neonatal rat mesencephalic V neurons
Neuropeptide Y modulates membrane excitability in neonatal rat mesencephalic V neurons
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神经肽 Y 调节新生大鼠中脑 V 神经元的膜兴奋性
DOI：
10.1002/jnr.24583
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发表时间：
2020
影响因子：
4.2
通讯作者：
Kogo Mikihiko
中科院分区：
文献类型：
--
作者：
Seki Soju;Tanaka Susumu;Yamada Saori;Tsuji Tadataka;Enomoto Akifumi;Ono Yudai;Chandler Scott H.;Kogo Mikihiko
神经肽Y （NPY）是具有强氧作用的神经肽之一。脑室内给药NPY可引起摄食量增加和摄食率的改变。除了在摄食行为中发挥作用外，NPY还对其他自发行为（如饲养和梳理）相关的神经系统有显著影响。在本研究中，我们研究了NPY对中脑V神经元（mesv）的直接影响。中脑V神经元是参与口腔运动反射、下颌节律运动以及咀嚼本体感觉的重要感觉神经元。新生儿Sprague - Dawley大鼠（P3 - 17）的冠状脑切片和Mes - V神经元的全细胞膜片钳记录。NPY可使大多数神经元的膜电位和诱导向内电流去极化。NPY的应用缩短了动作电位后超极化的持续时间，增加了重复放电时的平均尖峰频率。在维持电流注射反应中表现出节律性突发放电的神经元中，突发频率也增加。这些作用主要由Y1和Y5受体介导。
Neuropeptide Y (NPY) is one of a number of neuropeptides with powerful orexigenic effects. Intracerebroventricular administration of NPY induces increases in food intake and alters feeding rate. Besides it role in feeding behavior, NPY also has significant effects on neuronal systems related to other spontaneous behaviors such as rearing and grooming. In the present study, we examined the direct effects of NPY on mesencephalic V neurons (Mes V), which are important sensory neurons involved in oral motor reflexes and rhythmical jaw movements, as well as masticatory proprioception. Coronal brain slices were prepared from neonatal Sprague‐Dawley rats (P3‐17) and whole‐cell patch clamp recordings were obtained from Mes V neurons. Bath application of NPY depolarized the membrane potential and induced inward current in most neurons. Application of NPY shortened the duration of the afterhyperpolarization following an action potential, and increased the mean spike frequency during repetitive discharge. In those neurons which exhibited rhythmical burst discharge in response to maintained current injection, the bursting frequency was also increased. These effects were mediated predominately by both Y1 and Y5 receptors.