Human bone cell phenotypes differ depending on their skeletal site of origin.
Human bone cell phenotypes differ depending on their skeletal site of origin.
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人类骨细胞表型因其骨骼起源部位而异。
DOI：
10.1210/jcem.80.8.7629252
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
D. Baylink
中科院分区：
文献类型：
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作者：
C. Kasperk;J. Wergedal;D. Strong;J. Farley;K. Wangerin;H. Gropp;R. Ziegler;D. Baylink
这份报告描述了四名患者的研究中与骨骼部位相关的人类成骨细胞代谢的差异。Northern分析显示，在快速增殖和碱性磷酸酶(ALP)表达较少的下颌成骨细胞中，碱性成纤维细胞生长因子(BFGF)和胰岛素样生长因子II(IGF-II)等强有丝分裂生长因子的mRNA水平较低，而在缓慢增殖的髂骨成骨细胞中，碱性成纤维细胞生长因子(BFGF)和胰岛素样生长因子II(IGF-II)的mRNA表达水平较低。相反，转化生长因子-β(转化生长因子-β)mRNA在髂骨成骨细胞中比在下颌骨成骨细胞中更为丰富。此外，我们发现，基于这两个部位的数据，结构性转化生长因子βmRNA的水平与对外源性给予bFGF或IGF-II的反应之间存在一定的比例关系。在亚克隆的转化的人成骨细胞中也观察到了类似的生长特征和mRNA表达模式，这些细胞表达人成骨细胞分化标志物ALP的高水平和低水平。这些发现与1)人骨细胞表型与骨骼部位相关的差异一致；2)在碱性磷酸酶高表达的人骨细胞中，IGF-II和bFGF的表达减少，而转化生长因子β的表达和对bFGF和IGF-II的反应性增加。
This report describes skeletal site-related differences in human osteoblastic cell metabolism in studies of four patients. Northern analyses of the constitutive growth factor messenger ribonucleic acid (mRNA) expression pattern in mandibular and iliac crest-derived human osteoblastic cells (based on within-patient comparisons) revealed higher mRNA levels for strong mitogenic growth factors such as basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) in the rapidly proliferating and less alkaline phosphatase (ALP)-expressing mandibular osteoblastic cells compared to those in the lower bFGF and IGF-II mRNA levels in slowly proliferating iliac human osteoblastic cells exhibiting a higher ALP expression level. In contrast, transforming growth factor-beta (TGF beta) mRNA was more abundant in iliac human osteoblastic cells than in mandibular osteoblastic cells. Furthermore, we found that there was a proportionality, based on data from both sites, between the level of constitutive TGF beta mRNA and the response to exogenously administered bFGF or IGF-II. A comparable pattern of growth characteristics and mRNA expression was also observed in transformed human osteoblastic cells that had been subcloned in sublines expressing high and low levels of the human osteoblastic differentiation marker ALP. These findings are consistent with 1) skeletal site-related differences in human bone cell phenotypes, and 2) decreased IGF-II and bFGF expression and increased TGF beta expression and responsiveness to bFGF and IGF-II in human bone cells exhibiting a high ALP expression.