INHIBITION OF GROWTH OF GIARDIA-LAMBLIA BY DIFLUOROMETHYLORNITHINE, A SPECIFIC INHIBITOR OF POLYAMINE BIOSYNTHESIS
INHIBITION OF GROWTH OF GIARDIA-LAMBLIA BY DIFLUOROMETHYLORNITHINE, A SPECIFIC INHIBITOR OF POLYAMINE BIOSYNTHESIS
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DOI：
10.1111/j.1550-7408.1984.tb04308.x
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
MCCANN, PP
中科院分区：
文献类型：
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作者：
GILLIN, FD;REINER, DS;MCCANN, PP
二氟甲基鸟氨酸（DFMO）是一种特异性和不可逆的鸟氨酸脱羧酶抑制剂，该酶催化多胺生物合成途径的第一步。研究了DFMO对G.蓝氏杆菌、溶组织内阿米巴和迷走毛滴虫。芽孢杆菌生长在≥ 50 mg/L浓度下，DFMO抑制了兰氏杆菌。1.25培养物倍增时间随着DFMO浓度的增加而增加。如果亚精胺在添加DFMO后53小时内添加，则生长抑制被逆转;如果亚精胺稍后添加，则未观察到生长，表明最终寄生虫死亡。E. histolytica和T. 20 mM DFMO不能抑制2种不相关的人类粘膜寄生虫迷走线虫。很显然，G.兰布利亚
Difluoromethylornithine (DFMO) is a specific and irreversible inhibitor of ornithine decarboxylase, an enzyme which catalyzes the 1st step in the biosynthetic pathway of the polyamines. The effect of DFMO on the growth of G. lamblia, Entamoeba histolytica, and Trichomonas vaginalis was tested. Growth of G. lamblia was inhibited by DFMO at concentrations of .gtoreq. 1.25 mM. Culture doubling time increased with increasing DFMO concentration. Growth inhibition was reversed if spermidine was added within 53 h of addition of DFMO; no growth was observed if spermidine was added later, indicating eventual parasite death. The growth of E. histolytica and T. vaginalis, 2 unrelated mucosal-dwelling parasites of humans, was not inhibited by 20 mM DFMO. Apparently, polyamine biosynthesis from ornithine is required for growth of G. lamblia.